摘要
DREB(dehydration responsive element binding protein)类转录因子是AP2/EREBP(APETALA2/an ethylene-responsive element binding protein)转录因子家族的一个亚家族,拥有保守的AP2结构域,能够特异性地与抗逆基因启动子区域的DRE顺式作用元件相结合,在低温、干旱和盐碱等条件下调节一系列下游逆境应答基因的表达,是逆境适应中的关键性调节因子。拟南芥中与逆境相关的DREB转录因子可分为DREB1/CBF和DREB2两类,其中DREB1/CBF类成员主要参与低温胁迫应答反应,DREB2类成员则主要参与干旱胁迫应答反应。根据近十年来的报道,目前已从拟南芥、水稻、玉米、小麦、黑麦、大豆、西红柿和油菜等几十种植物中分离并鉴定出调控干旱、高盐及低温耐性的DREB基因,并利用这些基因得到了抗逆性增强的拟南芥、油菜、西红柿、小麦以及杨树等转基因植株。转基因结果表明DREB转录因子家族在双子叶植物,单子叶植物,草本植物及木本植物抗逆品种改良中均具有重要的应用价值。本文总结了十多年来特别是近五年来国内外相关研究进展,并对DREB转录因子的研究前景及其在木本植物良种选育中的研究趋势进行了展望。
Dehydration-responsive element binding （DREB） transcription factors with a typical AP2/EREBP DNA-binding domain might recognize and bind to DRE cis-element especially and finally activate a lot of stress-responsive genes when plants are under abiobitc stresses including dehydration, high salt and low-temperature. A rabidopsis DREB transcription factors mainly include DREB 1/CBF-type genes and DREB2-type genes that could function under low-temperature and dehydration, respectively. According to the published reports in decades, a vast number of DREB transcription factors have been isolated and characterized from a variety of plants such as Arabiclopsis, rice, maize, wheat, rye, Brassica napus, tomato and Festuca arundinacea. Over-expression of these DREB cDNAs in Arabidopsis, Brassica napus, tomato, wheat and poplar greatly could enhance the stress tolerance of these transgenic plants which indicated the importance of DREB transcription factors in both dicotyledon and monocotyledon plant, herbaceous and woody plant. This paper would be reviewed not only on roles of DREB 1 s and DREB2s in plant stress signaling but also on the advances of DREB in developing transgenic plants for stress tolerances. The future vision of DREB gene family were also discussed in this review based on the research achievements in the recent five years.
出处
《分子植物育种》
CAS
CSCD
2007年第F11期29-35,共7页
Molecular Plant Breeding
基金
国家自然科学基金(20571472
30271096
30371143
30460015)
国家"十一五"科技支撑计划课题(2006BAD03A01)
