摘要
目的:探讨胰岛素抵抗大鼠脂联素(APN)与核因子κB(NF-κB)抑制因子激酶(IKK)mRNA表达的关系。方法:采用高脂饲料喂养建立胰岛素抵抗大鼠模型,并用正常血糖-高血浆胰岛素钳夹技术评估。应用RT-PCR方法检测大鼠脂肪组织中APN和IKK mRNA的表达。结果:高脂饲料组大鼠的葡萄糖输注率明显低于基础饲料组[GIR60～120(0.76±0.28vs4.26±0.70)mg·kg-1·min-1,P<0.01];与基础饲料组相比,高脂饲料组大鼠脂肪组织APN mRNA的表达显著降低(A值APN/β-actin0.39±0.23vs1.26±0.30,P<0.05)、IKK mRNA的表达显著升高(A值IKK/β-actin0.99±0.10vs0.17±0.02,P<0.05),APN与IKK mRNA的表达相关(r=-0.83,P<0.05)。结论:胰岛素抵抗大鼠脂肪组织APN与IKK mRNA的表达显著负相关,提示IKK引起的NK-κB的活化可能与胰岛素抵抗时脂肪组织APN合成减少有关。
Objective：To investigate the relationship between adiponectin and nuclear factor-kappa B inhibitor kinase（IKK） mRNA expression in adipose tissue of insulin resistant rats.Methods：Insulin resistant rat model was established by high-fat diet feeding.Twenty male Wistar rats were randomly divided into two groups,each fed with either basic chow（NC,n = 10）or high-fat diet（HF,n = 10）for 10 weeks,then assessed by euglycemic-hyperinsulinemia clamp technique.The adiponectin and IKK mRNA expression in adipose tissue were determined with quantitative RT-PCR.Results：The glucose infusion rate（GIR） in HF group decreased.significantly compared with NC group [GIR60～120（0.76±0.28 vs 4.26±0.70） mg·kg-1·min-1,P 〈 0.01）.Compared with NC group,the adiponectin mRNA expression of adipose tissue decreased significantly（A value APN/β-actin 0.39±0.23 vs 1.26±0.30,P 〈 0.05） and the IKK mRNA expression increased significantly in HF group（A value IKK/β-actin 0.99±0.10 vs 0.17±0.02,P 〈 0.05）.There was negative correlation between adiponectin and IKK mRNA expression in adipose tissue of insulin resistant rats（r =-0.83,P 〈 0.05）.Conclusion：The adiponectin and IKK mRNA expression in adipose tissue of insulin resistant rats have obviously negative correlation,which implicates that NF-kappa B activation induced by IKK may be relative to synthetic decrease of adiponectin in adipose tissue when there is insulin resistance.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2007年第6期694-696,共3页
Journal of China Medical University
基金
辽宁省自然科学基金资助项目(20042094)
辽宁省教育厅高等学校科研基金资助项目(2004D192)
关键词
胰岛素抵抗
脂肪组织
脂联素
核因子κB抑制因子激酶
insulin resistance
adipose tissue
adiponectin
nuclear factor-kappa B inhibitor kinase
