摘要
以香果树（Emmenopterys henryi Oliv．）实生苗带芽茎段和叶片为外植体进行组织培养。结果表明，香果树带芽茎段愈伤组织的最适诱导培养基为含有1．0mg·L^-1～6-BA和0．01mg·L^-1 NAA的MS培养基，愈伤组织诱导率达100％；愈伤组织分化的最适培养基为添加2．0mg·L^-1KT和0．01mg·L^-1 NAA的MS培养基；在含有1．0mg．L^-1～6-BA和0．1mg·L^-1 NAA的MS培养基上，叶片诱导不定芽的效果较好，诱导率可达80％；在含有2．0mg．L^-1 KT和0．1mg·L^-1 NAA的MS培养基上，不定芽的增殖系数可达3—4；以含有1．5mg·L^-1 IBA的1／2MS培养基为生根培养基，香果树试管苗生根率达72．73％。移栽至大田后，香果树试管苗的成活率达到30％。
Tissue culture of Emmenopterys henryi Oliv. has been investigated using leaf and stem as explants. The results show that MS medium containing 1.0 mg· L^-1 6-BA and 0.01 mg · L^-1 NAA is suitable for inducing callus from stems, the induced rate reaches to 100%. MS medium containing 2.0 mg · L^-1 KT and 0.01 mg · L^-1 NAA is the best suitable medium for callus differentiation. On MS medium containing 1.0 mg · L^-1 6-BA and 0.1 mg · L^-1 NAA, inducement effect of adventitious shoot from leaf is better and the induced rate reaches to 80%. The perfect medium for adventitious shoot proliferation is the MS medium containing 2.0 mg · L^-1 KT and 0.1 mg · L^-1 NAA and the proliferation coefficient reaches to 3 - 4. Rooting rate of E. henry/ plantlets can reach to 72.73% by using 1/2MS medium containing 1.5 mg · L^-1 IBA as rooting medium. Survival rate of E. henry/ plantlets is about 30% after transplanting to field.
出处
《植物资源与环境学报》
CAS
CSCD
2008年第1期71-74,共4页
Journal of Plant Resources and Environment
基金
浙江省教育厅中青年教师资助项目(20020259)
关键词
香果树
组织培养
茎
叶
Emmenopterys henryi Oliv.
tissue culture
stem
leaf
