摘要
[目的]探讨新疆天山雪莲组培快繁技术。[方法]以新疆雪莲切除根部的无菌苗,或无菌苗的真叶,或者直接采集野生的新疆雪莲幼嫩部位作外植体,进行愈伤组织诱导培养及丛生芽分化、丛生芽增殖继代培养、生根培养和组培苗移栽试验。[结果]新疆天山雪莲外植体在MS+NAA 2 mg/L+6-BA 0.5 mg/L和MS+6-BA 1.0+NAA 0.1 mg/L+2,4-D 0.1 mg/L培养基上可诱导产生大量的丛生芽;在MS+6-BA 0.5 mg/L+NAA 2.0 mg/L和MS+6-BA 0.5 mg/L+NAA 0.01 mg/L培养基上交替培养,丛生芽继代快速增殖率达1.0∶3.5;组培苗在MS+NAA 0.2 mg/L培养基上诱导生根,生根率达83%;生根的雪莲组培苗经炼苗和移栽后成活,生长健壮。[结论]该研究建立的快繁技术为雪莲野生资源保护和产业化发展开拓了一条有效途径。
[Objective] The aim was to discuss the rapid propagation technique in tissue culture of Saussurea involucrata Kar.et Kir.from Tianshan mountain.[Method] With aseptic seedlings excised root part,their leaves of S.involucrata or the tender parts of wild S.involucrata collected directly as explants,the callus induction culture,clustered bud differentiation and proliferation subculture,rooting culture and transplant experiment of tissue-cultured seedlings were conducted.[Result] On the media MS＋NAA 2 mg/L＋6-BA 0.5 mg/L and MS＋6-BA 1.0＋NAA 0.1 mg/L ＋2,4-D 0.1 mg/L,the explants of S.involucrata from Tianshan mountain could be induced to generate plentiful clustered buds.In the alternate cultures on the media MS＋ 6-BA 0.5 mg/L＋NAA 2.0 mg/L and MS＋6-BA 0.5 mg/L＋NAA 0.01 mg/L,the rapid proliferation ratio of clustered bud subculture reached 1.0∶3.5.The tissue-cultured seedlings were induced to take root on the medium MS＋NAA 0.2 mg/L and the rooting rate reached 83%.The rooted tissue culture seedlings of S.involucrata could survive after domestication and transplant and grow healthily.[Conclusion] This rapid propagation technique set up in the study developed an effective approach for the protection and industrialized development of wild S.involucrata resources.
出处
《安徽农业科学》
CAS
北大核心
2008年第7期2743-2744,2746,共3页
Journal of Anhui Agricultural Sciences
关键词
新疆雪莲
组织培养
快速繁殖
Saussurea involucrata Kar.et Kir.
Tissue culture
Rapid propagation
