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PCR扩增法检测耐甲氧西林金黄色葡萄球菌的mecA基因 被引量:6

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摘要 目的应用mecA基因PCR扩增法检测耐甲氧西林金黄色葡萄球菌(MRSA)。方法对临床分离的84株金黄色葡萄球菌,应用mecA基因PCR扩增法鉴定MRSA,并与苯唑西林纸片扩散法和头孢西丁纸片扩散法进行比较。结果84株金黄色葡萄球菌中,41株金黄色葡萄球菌的mecA基因扩增呈阳性,其中有1株表现为对苯唑西林敏感;苯唑西林纸片扩散法检出42株阳性,其中有2株mecA基因呈阴性。头孢西丁纸片扩散法与PCR扩增法的试验结果完全符合。结论mecA基因PCR扩增法可以准确、快速判定MRSA。
出处 《广东医学》 CAS CSCD 北大核心 2008年第4期566-568,共3页 Guangdong Medical Journal
基金 湖南省自然科学基金(编号:06JJ4051)
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参考文献6

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