摘要
为了研究B7-1与CD40L共刺激途径在淋巴瘤的免疫治疗中的作用,探讨治疗淋巴瘤疫苗有效的作用方式,将A20淋巴瘤细胞株接种在BALB/c小鼠身上以构建荷瘤小鼠模型,将B7-1与CD40L表达载体单独或联合导入肿瘤内,以PBS和空载体作为对照,观察肿瘤生长的情况;应用肿瘤病理切片和HE染色技术观察肿瘤组织形态及淋巴细胞浸润情况,采用CCK-8试剂盒检测荷瘤小鼠脾CTL杀伤效应。结果显示,瘤内注射B7-1或CD40L表达载体可导致肿瘤生长延缓或体积缩小,两者联合注射较对照组和单独注射组的肿瘤消退效应明显增强。肿瘤形态学观察表明,治疗小鼠肿瘤局部有炎性细胞浸润并伴有大面积的坏死,肿瘤局限化;CCK8试剂盒检测显示小鼠脾CTL杀伤能力增强。结论:B7-1与CD40L疫苗对淋巴瘤具有治疗效应,两者联合治疗的效果优于单一治疗。质粒载体瘤内注射可作为一种安全有效的肿瘤疫苗作用方式。
The aim of study was to investigate the synergetic effect of B7-1 and CD40L co-stimulating pathway in the immunotherapy for lymphoma and to explore the effective manner of tumor vaccine for treating lymphoma, The lymphoma cell line A20 cells were inoculated into BALB/c mice as to establish A20-bearing mice model, the BT-1 and CD40L expression vector were alone or in combination directly injected into lymphoma of mice model, the PBS, vector pcDM8 and pcDNA3.1 were selected as controls so as to observe tumor growth. The pathological section and HE staining of tumor tissue were performed to observe the histological characteristics and the cell infiltration of lymphoma, the CCK-8 detection kit was used to analysis the splenic CTL cytotoxicity. The results showed that the intratumor injection of B7-1 and CD40L resulted in reduction of tumor size. Morphological observation of tumor revealed inflammatory cell infiltration in the tumors, massive necrosis and localization of tumor. CCK-8 kit detection indicated significant enhancement of splenic CTL cytotoxicity, the effect of B7-1 combined with CD40L was stronger than that of B7-1 or CD40L alone. It is concluded that B7-1 and CD40L show immunotherapeutic effect on lymphoma, and the effect becomes stronger when they are combined in treating lymphorna. Meanwhile, the intratumor injection may be considered as a safe and effective way for tumor vaccine.
出处
《中国实验血液学杂志》
CAS
CSCD
2008年第2期317-321,共5页
Journal of Experimental Hematology
基金
国家自然科学基金,编号30371579
