摘要
目的探讨萜类物质YK-1和YK-2体外抗细菌和抗真菌作用。方法用试管倍比稀释法测定两种萜类物质对常见病原性细菌和病原性真菌的最小抑菌浓度(Minimum inhibitory concentration,MIC)及最小杀菌浓度(Minimum bactericidal concent ration,MBC),判断其抗菌活性。结果YK-1对金黄色葡萄球菌、大肠埃希菌、绿脓杆菌、白色念珠菌和石膏样小孢子菌的MIC分别为0.20、0.78、0.39、0.39和0.78mg/ml,MBC分别为0.78、1.56、1.56、0.78和1.56mg/ml;YK-2对上述病原微生物的MIC依次为6.39、0.39、0.39、0.39和0.20mg/ml,MBC依次为0.39、0.78、0.78、0.78和0.39mg/ml。结论2种萜类物质均有抗菌作用,但对不同病原微生物的抗菌活性存在差异。
Objective To explore antibacterial effects of terpenoides YK-1 and Yk-2 on common pathogenic bacteria and pathogenic fungi in vitro. Methods Testing minimum inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） of YK- 1 and YK-2 to Staphylococcus aureus , Escherichia coli , Pseudornonas aeruginosa , Candida albicans, and Microsporurn flavescens by serial tube dilution method. Results MICs of YK-1 to S. aureus, E. coli, P. aeruginosa, C. albicans, and M. flavescens were 0.20, 0.78, 0.39, 0.39 and 0.78 mg/ml respectively, MBCs were 0.78, 1.56, 1.56, 0. 78 and 1.56 mg/ml respectively. MICs of YK-2 to the bacteria and fungi as above were 6.39, 0.39, 0.39, 6.39 and 0.20 mg/ml respectively, MBCs were 0.39, 0.78, 0.78, 0.78和0.39 mg/ml. Conclusion Terpenoides YK-1 and Yk-2 show antibacterial activities agaist S. aureus, E. coli, P. aeruginosa, C. albicans, and M. flavescens, but there are different to different pathogenic organisms.
出处
《中国病原生物学杂志》
CSCD
2008年第3期176-177,共2页
Journal of Pathogen Biology
