摘要
目的:研究左归丸和右归丸对实验性变态反应性脑脊髓炎(EAE)大鼠神经髓鞘和轴突再生的影响。方法:应用髓鞘碱性蛋白(MBP)与完全福氏佐剂(CFA)按1∶1(体积比)制成抗原,给动物两后足皮下注射MBP抗原免疫,建立大鼠EAE模型。以醋酸泼尼松作对照,利用髓鞘染色及免疫组织化学染色观察各组大鼠造模后14,28 d大脑和脊髓髓鞘和轴突损伤及再生的变化。结果:左归丸成右归丸干预后大鼠髓鞘脱失程度均较模型组明显减轻,MBP及神经丝蛋白(NF200)表达显著高于模型组(P<0.05或0.01)。结论:左归丸和右归丸能够修复EAE大鼠神经髓鞘和轴突的损伤,可能是其促进神经康复的分子机制之一。
Objective： To explore effects of Zuo Gui Pill and You Gui Pill on remyelination and axonal regeneration in experimental allergic encephalomyelitis （EAE） model rat. Methods： The rats were injected antigen containing myelin basic protein（MBP） and Freund＇s Adjuvant Complete（CFA）in the same volume in both rat postpeds. Asa control of prednisone, the injury and regeneration of myelin and axon in rat brain and spinal cord were observed by using myelin staining and immunohistochemistry method after the rats model were made at 14 and 28 days. Results：The injury of myelin and axon were reduced by treated with Zuo Gui Pill and You Gui Pill in EAE rat, Both of MBP and neurofilament protein（NF200） expressions were increased significantly in the treatment groups of Zuo Gui Pill and You Gui Pill （P 〈 0.05 or 0.01 ） compared with the model group. Conclusions：Zuo Gui Pill and You Gui Pill Can restorate the nervous myelin and axonal injury which may be the mechanism of tonifying-kidney formulae in promoting rehabilitation in rats with EAE.
出处
《中国实验方剂学杂志》
CAS
2008年第4期42-45,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
北京市中医药科技项目(2003Ⅲ-21,JJ2005-8)
北京市教育委员会科技发展计划项目(KM2007100025015)
北京市自然基金(7062022)
国家自然科学基金(30640069)
关键词
左归丸和右归丸
实验性变态反应性脑脊髓炎
髓鞘再生
轴突再生
Zuo Gui Pill and You Gui Pill
experimental allergic encephalomyelitis
remyelination
axonal regeneration
