摘要
目的:观察甘露消毒丹对温病湿热证大鼠细胞内毒素特异性受体(LBP mRNA),(CD14 mRNA)及(NF-κBp65)表达变化的动态干预,深入探讨清热化湿方的治疗机制。方法:分为正常组、湿热模型组(高脂+高温高湿+大肠杆菌)、清热化湿组;采用逆转录-聚合酶链反应技术(RT-PCR)检测肝巨噬细胞LBP mRNA,CD14 mRNA,免疫组化技术检测肝巨噬细胞NF-κBp65活化。结果:模型组在感染6,12,24 h等不同时相点LBP mRNA,CD14 mRNA,NF-κB表达逐渐非常显著增强,且不同时相点之间比较均有显著性差异,说明随着病程的发展,湿热模型LBP mRNA,CD14 mRNA,NF-κB表达逐渐增强。治疗组6,12,24 h等不同时相点比较,LBP mRNA,CD14 mRNA表达减弱,具有非常显著性差异;24 h时相点治疗组与模型组比较LBP mRNA,CD14 mRNA表达减弱有非常显著性差异,但仍未恢复正常,说明LBP mRNA,CD14 mRNA遏制衰减是一个缓慢的过程。而治疗组NF-κB表达6,12,24 h各时相点间比较无显著性差异;且与正常组比较NF-κB激活均无显著性变化。结论:甘露消毒丹对温病湿热证大鼠肝巨噬细胞LBP mRNA,CD14 mRNA及NF-κB的激活具有较好的干预调控作用,能中止炎症介质的转录,限制急性炎症反应。
Objective：To observe the dynamic interference in LBP mRNA,CD14 mRNA, NF-κBp65 on the cell endotoxin（ET） specific receptors of damp-heat syndrome model rats treated by Ganlu Xiaodu Dan, furthe to explore the therapy mechanism of Qingre Zaoshi Prescription. Methods： Animal were divided into normal group, model group （high fat diet ＋ high temperature and high humidity ＋ colon bacillus ）, Qingre Zaoshi group randomly, the expression of LBP mRNA, CD14 mRNA, in liver macrophage was semiquantitated by reverse transcription-polymerase chain reaction（ RT- PCR）, the activation of nuclear factor-kappa Bp65 （NF-κBp65） in liver macrophage was detected by immuno-histochemical methods. Results ： At 6 h, 12 h and 24 h action spots, the expressions of LBP mRNA, CD14 mRNA, NF-κBp65 in model group was remarkably increased compared with those of normal group;And at 6 h, 12 h and 24 h,the above indicators had significant difference compared with each other. The result indicated that during the development of the course of disease, the expression of LBP mRNA, CD14 mRNA, NF-κBp65 in model group was increased gradually. The expression of LBP mRNA, CD14 mRNA, in treatment group was decreased, and had significant difference between 6 h and 12 h and 12 h and 24 h. At 24 h, the expression of LBP mRNA, CD14 mRNA in treatment group was decreased, and had significant difference compared with model group. The results suggested that attenuation of LBP mRNA, CD14 mRNA, is a slow course. Conclusions：It was suggested that Ganlu Xiaodu Dan has interference in LBP mRNA, CD14 mRNA, NF-κBp65 ir liver macrophage of damp-heat syndrome of seasonal febrile disease rats.
出处
《中国实验方剂学杂志》
CAS
2008年第4期56-59,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
巨噬细胞
肝
湿热证
大鼠模型
甘露消毒丹
macrophage
liver
damp-heat syndrome
rat model
Ganlu Xiaodu Dan
