摘要
为了探讨兔斯氏艾美耳球虫(E.stiedai)感染兔肝脏诱导型一氧化氮合酶(iNOS)活性和血清NO浓度变化以及NO对兔球虫的抑制或杀伤作用,以腹腔注射途径给予球虫感染家兔一氧化氮合酶(NOS)底物L-精氨酸(L-Arg)和iNOS抑制剂L-氨基胍(L-AG),对家兔球虫感染时肝脏iNOS活性、血清NO浓度、每克粪便的球虫卵囊数(OPG)、肝的剖检和镜下病变及其病变计分进行了研究。试验结果表明:①家兔感染E.stiedai后,肝匀浆的iNOS活性和血清中NO浓度逐渐升高,感染对照组、添加L-Arg组和添加L-AG组3组均在感染后第12天达到最高值,而后逐渐下降,于23 d左右下降到感染前的水平;②L-Arg可增强iNOS的活性,促进体内大量生成NO,进而提高家兔对球虫的抑制作用,减轻球虫感染引起的病变;L-AG则降低iNOS的活性,使体内生成的NO减少,从而加重球虫感染引起的病变。结果提示:NO及iNOS确实参与了家兔E.stiedai的感染过程,并且NO在球虫感染过程中起到了抑制或杀伤兔球虫的作用。
To probe the activity of inducible nitric oxide synthase （iNOS） in liver , the concentration of nitric oxide （NO） in serum and its inhibition or killness on coccidia in Eimeria stiedai infected rabbits, the infected rabbits were injected with the substrate of nitric oxide synthase L-arginine （L-Arg） and the inhibitor of inducible nitric oxide synthase L-aminoguanidine （L-AG） through the abdominal cavity. Then the activity of iNOS in liver , the concentration of NO in serum,the coccidian oosysts per gram feces （OPG）, the anatomic structures and microscopic pathological changes of rabbits＇ livers and their scores were studied. The results showed that ：① The activity of iNOS of the uniform slurry of the liver and the concentration of NO in serum increased gradually after infection, and that in control group, L-arginine group, and L-AG group reached the top at 12 days after infection, then decreased gradually and reached the level before infection at ～23 days; ②L-Arg can enhance the activity of iNOS and the release of large amount of nitric oxide, consequently it inhibit or kill Eimeria stiedai and ease the pathological changes caused by Eimeria stiedai ; while L-AG decreased the activity of iNOS and reduce NO production in vivo, so that it aggravates the pathological changes caused by Eimeria stiedai. The results showed that NO and iNOS participate in the infection process of Eimeria stiedai in rabbit and NO plays an role of inhibition or killness on coccidia of E. stiedai.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2008年第4期483-487,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
山东省自然科学基金资助项目(Y2003D04)
