期刊文献+

不同品种绵羊TGF-β1基因单核苷酸多态性及其与繁殖力关系的研究 被引量:4

The Analysis for the Single Nucleotide Polymorphism of TGF-β1 and Its Association with Reproduction in Different Breeds Sheep
下载PDF
导出
摘要 以小尾寒羊、滩羊、同羊、欧拉羊共计196头为研究材料,采用PCR-SSCP技术,对绵羊TGF-β1基因6-7外显子区间内的805 bp序列进行多态性分析,发现了一个多态位点。经克隆测序分析发现,第6内含子区内存在一个突变,该突变位点为第7外显子上游的第294位碱基处缺失了AGAC(序列:gi76871756中的14201位)。对不同绵羊群的基因型和等位基因频率统计结果表明,多胎品种小尾寒羊以AB基因型为主。χ2检验结果表明,单胎品种滩羊、同羊、欧拉羊以BB基因型为主,所有品种都处于Hardy-Weinberg平衡状态。 A single nucleotide polymorphism (SNP) of sheep 805 bp sequence in 6th intron region of transforming growth factor/31 (TGF-β1) gene was identified in 196 sheep among Small-tailed Han,Tong,Tan and Oula sheep by PCR-SSCP, which declared one mutation point. Comparative sequencing analysis of cloned products revealed a AGAC deletion at upstream 294 of extron 7 of TGF-β1 gene (site 14 201 in gi76871756). The statistical results of genotype and allele frequency in different breeds showed that the genotype AB dominated in the Small-tailed Han sheep. The genotype BB was in majority in low-fecudity sheep. The results of x^2 test indicated that all the populations were in Hardy-Weinberg equilibrium.
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2008年第4期503-507,共5页 ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金 国家自然科学基金项目(30270949) “863”计划项目(2006AA10Z199)
关键词 转化生长因子Β1 PCR—SSCP 单核苷酸多态性 绵羊 TGF-β1 PCR-SSCP single nucleotide polymorphism(SNP) sheep
  • 相关文献

参考文献13

  • 1RIDDHISH S, BRAD R, CAROLYN K H, et al. Allelic diversity in the TGFB1 regulatory region: characterization of novel functional single nucleotide polymorphisms[J]. H urn Genet, 2006,119 : 61-74.
  • 2MCNATTY K P,JUENGEL J L,WILSON T,et al. Oocyte derived growth factors and ovulation rate in sheep[J]. Reproduction, 2003,64 (Suppl) : 1-13.
  • 3MONTGOMERY G W ,GALLOWAY S M ,DAVIS G H,et al. Genes controlling ovulation rate in sheep [J]. Reproduction, 2001,121 : 843-852.
  • 4RON W P, BABITA S, MELISSA J,et al. Immunohistochemical localization of TGF-b1, TGF-b2 and TGF-b3 in the mouse embryo: expression patterns suggest multiple roles during embryonic development [J]. Cell Biology, 1991, 115:1 091-1 105.
  • 5JEFFREY V M, LISA A S, CRAIG J T, et al. Transforming growth factor t3 expression in the porcine ovary: evidence that theca cells are the major secretory source during antral follicle development [J]. Biology of Reproduction, 1996, 54 : 485-496.
  • 6JESSE GORE A, DANIEL P P, WILLIAM L M, et al. Differential regulation of follicle stimulating hormone by activin A and TGFB1 in murine gonadotropes [J]. Reproductive Biology and Endocrinology, 2005,3:73.
  • 7GEROLD U. Profiling molecular targets of TGF-bl in prostate fibroblast-to-myofibroblast transdifferentiation [J]. Mechanisms of Ageing and Development,2005, 126:59-69.
  • 8萨姆布鲁克J 拉塞尔DW著 黄培堂 译.分子克隆实验指南[M].北京:科学出版社,2002.481-482.
  • 9武艳萍,傅金恋,李宁,王晓凤,卢纪和,王爱国.不同猪种TGF-β1基因单核苷酸多态性分析[J].中国畜牧杂志,2005,41(4):11-13. 被引量:7
  • 10MOCHAMMAD I, EDDY S, MOHAMMAD S R. Decreased TGF-β1 and IGF-1 protein expression in rat embryo skull bone in folic acid-restricted diet[J]. Nutritional Biochemistry, 2006,17 (1) : 51-56.

二级参考文献78

  • 1Landschulz WH, Johnson PF, McKnight SL. The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins. Science 1988,240:1759-1764.
  • 2Glover JN, Harrison SC, Crystal structure of the heterodimeric bZIP transcription factor c-Fos-c-Jun bound to DNA. Nature 1995,373:257-261.
  • 3Alber T. Structure of the leucine zipper. Curr Opin Genet Dev 1992,2:205-210.
  • 4Ryder K, Lanahan A, Perez-Albuerne E, Nathans D.jun-D: a third member of the jun gene family. Proc Natl Acad Sci USA 1989, 86:1500-1503.
  • 5Hartl M, Hutchins JT, Vogt PK. The chicken junD gene and its product. Oncogene 1991,6:1623-1631.
  • 6Hirai SI, Ryseck RP, Mechta F, Bravo R, Yaniv M.Characterization of junD: a new member of the jun proto-oncogene family. EMBO J 1989,8:1433-1439.
  • 7Nishina H, Sato H, Suzuki T, Sato M, Iba H. Isolation and characterization of fra-2, an additional member of the fos gene family. Proc Natl Acad Sci USA 1990,87:3619-3623.
  • 8Aronheim A, Zandi E, Hennemann H, Elledge SJ,Karin M. Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions.Mol Cell Biol 1997,17:3094-3102.
  • 9Angel P, Karin M. The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation.Biochim Biophys Acta 1991, 1072:129-157.
  • 10Angel P, Imagawa M, Chiu R, Stein B, Imbra RJ,Rahmsdorf HJ, Jonat C, Herrlich P, Karin M. Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor.Cell 1987, 49:729-739.

共引文献193

同被引文献41

  • 1武艳萍,傅金恋,李宁,王晓凤,卢纪和,王爱国.不同猪种TGF-β1基因单核苷酸多态性分析[J].中国畜牧杂志,2005,41(4):11-13. 被引量:7
  • 2钱剑,刘国文,付世新,王哲.铜对仔猪关节软骨细胞TGF-β mRNA基因表达的影响[J].中国兽医学报,2005,25(5):508-510. 被引量:3
  • 3Jennifer L J,Adrian H B,Karen L R. The role of transforming growth factor beta(TGF-beta) during ovarian follicular development in sheep[J].Reproductive Biology and Endocrinology,2004,(02):78.
  • 4Shi Y,Massagye J. Mechanisms of TGF-beta signa ling from cell membrane to the nucleus[J].Cell,2003.685-700.
  • 5Santoso M I E,Rohman M S. Decreased TGF-β1 and IGF-1 protein expression in rat embryo skull bone in folic acid restricted diet[J].Journal of Nutritional Biochemistry,2006.51-56.
  • 6May J V,Stephenson L A,Turzcynski G J. Transforming growth factor β expression in the porcine ovary:evidence that theca cells are the major secretory source during antral follicle development[J].Biology of Reproduction,1996.485-496.
  • 7Gore A J,Philips D P,Miller W L. Differential regulation of follicle stimulating hormone by activin A and TGFβI in murine gonadotrope[J].Reproductive Biology and Endocrinology,2005,(03):73.
  • 8Antoni X,Alejandra M A,Sergio L. Transforming growth factor-β1 gene polymorphisms are associated with disease progression in idiopathic pulmonary fibrosis[J].American Journal of Respiratory and Critical Care Medicine,2003.431-435.
  • 9Mcnatty K P,Juengel J L,Wilson T. Oocyte derived growth factors and ovulation rate in sheep[J].Reproduction,2003.339-351.
  • 10Sutton A B,Canfield A E,Schor S L. Endoglin modulates cellular responses to TGF-β1[J].Journal of Cell Biology,1996.1109-1121.

引证文献4

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部