摘要
邻二氮菲分光光度法测定黄芪中铁的溶出率,研究了不同溶剂对铁的溶出率的影响。检测波长510nm,在0.1—6μg·mL-1的范围内符合比耳定律,相关系数r=0.9995,检出限为13.7μg·L-1,方法回收率为96.1%—102.3%,精密度为0.38%—2.31%。测定结果表明,分别以水、不同浓度乙醇、不同浓度盐酸作溶剂,灰化后再用水提取铁的溶出率最高,达到44.0%,直接用酸、水、乙醇作溶剂,溶出率依次降低。本法简便、易行,且重现性好。
The dissolution rate of iron in astragli radix was studied by spectrophotometry. The effect of different extraction reagent was also studied. Beer＇s law was obeyed over the range of 0. 1-6μg·mL^-1 at 510 nm. The limit of detection and relative standard deviations are 13. 7μg·L^-1 and 0.38%-2.31%,respectively. The recovery is of 96.1% -102.3%. Several extraction reagents such as water,alcohol,and acid were used and the maximum extracting rate （44.0%） could be obtained if astragli radix was processed by water extraction after ashed while it decreased by acid,water,alcohol extraction respectively. The method is rapid ,simple and stable.
出处
《光谱实验室》
CAS
CSCD
2008年第2期205-208,共4页
Chinese Journal of Spectroscopy Laboratory
关键词
分光光度法
邻二氮菲
黄芪
铁
溶出率
Spectrophotometry, 1,10-Phenanthroline,Astragli Radix,Iron,Extracting Rate.
