摘要
目的运用组织培养技术对黄瑞香的愈伤组织进行诱导,对其二次代谢产物的化学成分进行系统研究。方法用组织和细胞培养技术进行愈伤组织培养,用制备高效液相、液-液萃取、硅胶柱层析、制备薄层层析法分离,波谱法鉴定结构。结果从黄瑞香的二次代谢产物中分离得到了5个化合物单体,分别为瑞香酮、7-羟基香豆素、7-羟基8-O-β-D-葡萄糖香豆素、西瑞香素和丁香苷。结论首次成功诱导得到了黄瑞香的愈伤组织,确定了其最佳诱导条件;首次从黄瑞香的二次代谢产物中分离得到了C6-C5-C6型化合物。
Objective To induce D. giraldii callus by using plane tissue culture technique and to systematically study the chemical components of the D. giraldii cultured cells. Methods High performance liquid chromatography （HPLC）, Gel column chromatography, liquid-liquid extraction, silica gel thin-layer chromatography were used to isolate the compositions and the spectrum was used to identify the structure of the compositions. Results Five compounds were isolated and identified as follows： daphneolone （ Ⅰ ）, umbelliferone （ Ⅱ ）, daphnetin-8-O-β-D-glucoside （ Ⅲ ）, daphnoretin （ Ⅳ ） and syringin（ Ⅴ ）. Conclusion It was the first time using plant cell culture technology to obtain D. giraldii callus and the best inductive condition was established. The C6-C5-C6 carbon skeleton components were isolated from D. giraldii culture cells for the first time.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2008年第3期199-201,共3页
Journal of Beijing University of Traditional Chinese Medicine
关键词
黄瑞香
组织培养
二次代谢产物
化学成分
D. giraldii
culture of callus
secondary metabolites
chemical constituents
