摘要
目的:建立舒肝活血胶囊的质量标准。方法:采用薄层色谱法(TLC)对舒肝活血胶囊中的柴胡、黄连、黄柏、黄芩进行定性鉴别,以高效液相色谱法(HPLC)对其主要药效成分黄芩苷和盐酸小檗碱进行含量测定。结果:舒肝活血胶囊中柴胡、黄连、黄柏、黄芩的供试品色谱中,在与对照品色谱相应的位置上,显相同颜色的斑点;黄芩苷进样量在0.633～3.800μg范围内与峰面积积分值呈线性关系(r=0.9996),小檗碱进样量在0.128～0.768μg范围内与峰面积积分值呈线性关系(r= 0.9995)。结论:本方法简便、准确、灵敏、重现性好,可用于本品的质量控制。
Objective： To establish the quality criteria of shuganhuoxue capsule. Method： TLC was employed to make qualitative determination of radix bupleuri, rhizoma coptidis, cortex phuollodendri chinensis, radix scutellariae in Shuganhuoxue capsule, and HPLC was Used to determine content of baicalin and berberine in its chief medicine material. Result： The spots of radix bupletturi, rhizoma coptidis, cortex phuollodendri chinesis, radix scutellariae in TLC were same color with those in the chromatograms of control articles. The calibration curves of two ingredients were linear within range of 0.633μg- 3. 800μg（ r = 0.999 6, baicalin ）, 0. 128 μg 0.768 μg（ r = 0.999 5,berberine）, respectively. Conclusion： The method is simple, accurate, sensitiveness and highly reproductive , which can be used for quality control of this preparation.
出处
《中国药师》
CAS
2008年第4期427-429,共3页
China Pharmacist
