摘要
目的:探讨幽门螺杆菌(H pylori)刺激人胃癌细胞SGC-7901后,对细胞形态,迁移以及细胞骨架的影响.方法:从人胃黏膜组织中分离培养H pylori,将得到的H pylori菌体超声裂解,用超声提取物刺激SGC-7901细胞,直接观察SGC-7901细胞形态的变化:细胞迁移活动用Boyden迁移槽和划线法分析;用phalloidin对细胞内F-actin进行荧光染色,研究其对细胞骨架的作用.各组间细胞迁移数量比较用单因素方差分析,组间两两比较用SNK检验:对照组和刺激组细胞发生蜂鸟表型的百分率进行t检验.结果:H pylori菌体超声提取物刺激人胃癌细胞SGC-7901后,细胞的活动性增强,高浓度和低浓度刺激组与对照组相比细胞迁移数明显提高(F=12697.314,P<0.01),两两之间相比也具有统计学意义(P<0.01).受刺激后蜂鸟表型细胞数较对照组大大提高(t=29.626,P<0.01).对照组细胞仅有少量应激纤维形成,H pylori刺激10 min后,应激纤维增加.结论:H pylori可诱导胃癌细胞SGC-7901发生形态改变,促进其迁移,并且增加细胞内细胞骨架的形成.
AIM： To investigate the effect ot Helicobacter pytori （H pylori） on the morphology, motility and cytoskeleton of human gastric cancer SGC-7901 ceils.
METHODS： H pylori were isolated from human gastric mucosa and cultured on a solid agar medium. The SGC-7901 cells were treated with the sonication extract to observe the morphological alteration, cell motility and cytoskeleton. Cell motility was examined by Boyden chamber motility assay and scrape motility assay. Cytoskeleton was observed by F-actin phalloidin fluorescent staining. The number of migrated SGC-7901 cells in the Boyden chamber was compared among three groups by one-way ANOVA, and the numbers between each two groups were compared by SNK test. The t test was used to compare the percentage of hummingbird-phenotype SGC-7901 cells between the stimulated group and control group. RESULTS： The sonication extract enhanced SGC-7901 cell motility, induced the appearance of hummingbird-phenotype, and increased cy- toskeleton. The Boyden chamber motility assay showed that the number of migrated SGC-7901 cells stimulated by high and low concentration of H pylori extract was significantly increased in comparison with that in the control group （F = 12697.314, P 〈 0.01）, and there was also a significant difference between the two groups （P 〈 0.01）. The percentage of hummingbird-phenotype SGC-7901 cells in the stimulated group was significantly higher than that in the control group （t = 29.626, P 〈 0.01）. Cytoskeleton was just a little in the control group, but increased a lot after 10-min stimulation.
CONCLUSION： H pylori can induce morphological alteration of human gastric cancer SGC-7901 cells, enhance their motility and formation of cytoskeleton in the cells.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第9期919-923,共5页
World Chinese Journal of Digestology
基金
江苏省社会发展项目
No BS2006041~~
关键词
幽门螺杆菌
胃癌细胞
蜂鸟表型
迁移
细胞骨架
Helicobacter pylori
Gastric cancer cells
Hummingbird-phenotype
Motility
Cytoskeleton
