摘要
目的:研究Delta-like 4(DLL4)在胃癌中的表达及其与血管生成的关系.方法:采用免疫组化En Vision法检测胃癌组织芯片中DLL4的表达,用CD34进行微血管内皮细胞染色,计算微血管密度(MVD),分析其相关性.结果:DLL4在胃癌中的表达明显高于正常胃黏膜(85.9% vs 35.3%,P<0.01).DLL4的高表达与胃癌的转移(r=0.612,P<0.01)和胃壁浸润深度(r=0.482,P<0.01)呈正相关,与胃癌的组织病理及Borrmann分型无关.胃癌组织MVD明显高于正常胃黏膜组织(66.5±18.6 vs 34.2±16.4,P<0.01).MVD值与胃癌的组织病理分型(r=0.506,P<0.01)和转移有关(r=0.426,P<0.01),与胃癌胃壁浸润深度和Borrmann分型无明显相关性.DLL4表达阳性组的MVD指数明显高于DLL4表达阴性组(70.5±16.2 vs 32.5±10.4,P<0.01),DLL4表达与MVD呈正相关(r=0.521.P<0.01).结论:DLL4表达促进血管分化,对胃癌的转移、浸润起重要作用.
AIM： To explore the expression of Delta-like 4 （DLL4） and its relations with the angiogenesis in gastric cancer.
METHODS： EnVision immunohistochemical staining was used to detect the expression of DLL4. The microvessel density （MVD） was calculated after CD34 staining for microvessel endothelium and the correlation between their roles and the pathological behavior of gastric carcinoma was investigated.
RESULTS： The expression of DLL4 in gastric cancer was significantly higher than that in normal gastric mucosa （85.9% vs 35.3%, P 〈 0.01）. The over-expression of DLL4 in gastric cancer was positively related to the metastasis （r = 0.612, P 〈 0.01） and invasion depth （r = 0.482, P 〈 0.01）,while not related to the histological types and Borrmann types. The MVD in gastric cancer tissues was notably higher than that in normal mucosal tissues （66.5 ± 18.6 vs 34.2 ±16.4, P 〈 0.01）. MVD was correlated with the histological types （r = 0.506, P 〈 0.01） and metastasis （r = 0.426, P 〈 0.01）, while it was not correlated with the depth of invasion and Borrmarm types. The MVD of DLL4-positive group was higher than that of DLL4-negative one （70.5 ± 16.2 vs 32.5 ±10.4, P 〈 0.01）, and the expression of DLL4 and MVD were positively correlated （r = 0.521, P 〈 0.01）.
CONCLUSION： DLL4 expression may play an important role in gastric carcinogenesis by promoting vascular differentiation, tumor invasion and metastasis.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第9期956-961,共6页
World Chinese Journal of Digestology
