摘要
将100只1日龄艾维茵肉仔鸡随机分成2组,即正常对照组、试验组(中药组),分别测定法氏囊指数、法氏囊细胞凋亡率以及外周血CD4+、CD8+T淋巴细胞转化率、法氏囊细胞周期时相、Bcl-2和Caspase-3基因的蛋白表达水平。结果表明:试验组的法氏囊指数14日龄、21日龄显著升高;试验组14、21日龄法氏囊细胞凋亡率明显降低,差异显著(P<0.05);细胞周期时相发生变化,G1期细胞百分比数量明显减少,G2期细胞百分比数量明显增加,差异均显著(P<0.05);外周血液中T淋巴细胞转化率明显提高,差异显著(P<0.05);由此看来,中药能促进法氏囊相对重量的增加,T淋巴细胞的转化向CD4+偏移,使细胞免疫功能增强。
Effect of herbal medicines on immunore gulation of heat-stressed broilers were studied in the experiment. 100 one-day Avian broilers were randomly divided into two groups,the control group and the treatment group respectively on the 14^th,21^th and 28^th. The growth index,cell apoptosis ratio,the distribution of cell cycle nf fabricius bursa,T lymphocyte subsets percentage nf peripheral blood,expression of Caspase-3 and Bcl-2 in bursa cells were measured. The results showed that growth index of the treatment broiler had remarkablv risen and cell apoptosis ratio had significantly decreased than control group on the 14^th and 21^th respectively（P〈0.05）. At the same time G1 and G2 ratios of cell cycle had obviously changed in treatment broiler than control group respectively on the 14^th and 21^th（p〈0.05）,this change indicated that the of G1 ratio of cell cycle had risen and G2 ratio of cell cycle had significantly decreased, T lymphocyte subsets percentage of peripheral blood had significantly risen than control group on the 14^th and 21^th respectively（P〈0,05）. It was concluded from above the results that herbal medie, ines could
出处
《中国家禽》
北大核心
2008年第7期22-24,共3页
China Poultry
基金
河北省邯郸市科技攻关项目(0405-2)
关键词
肉仔鸡
中药
热应激
免疫调节
broiler
herbal medicine
heat stress
immune regulation
