摘要
根据猪生殖与呼吸综合征病毒（PRRsV）美洲株ATCCVR-2332保守区ORF6和ORF7的基因序列，设计了1条37bp的寡核苷酸探针，末端用生物素标记。应用原位杂交方法对人工感染PRRSVSC-1株的28日龄仔猪进行了病毒核酸分布规律的研究。结果显示，感染后7～28d于肝、脾、肺、肾、扁桃体、胸腺、肺门淋巴结、肠和大脑检测到阳性杂交信号，其中肺门淋巴结的阳性信号一直较强，脾的阳性信号逐渐增强，肺的阳性信号较弱，心脏未见阳性杂交信号。
According to the conserve ORF6 and ORF7 gene sequences of the North American strain ATCC VR-2332 of porcine reproductive and respiratory syndrome virus（PRRSV）,a 37 bp oligonucleotide probe was designed,and labeled with biotin. The distributions of PRRSV in the 28-day-old-piglets at different time points post-infection were analyzed by using in situ hybridization. The result showed that positive signals were found from day 7 to 28 post-infection in tonsil, hilar node, thymus, spleen, lung, kidney, and cerebrum. Strong positive in situ hybridization signal was found in hilar node. The positive signals in spleen increased gradually post-infection. The weak positive signals were found in lung. Signal in heart was negative.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2008年第4期278-282,共5页
Chinese Veterinary Science
基金
高等学校科技创新工程重大项目培育资金项目(706050)
国家科技攻关计划项目(2004BA901A03)
四川省科技支撑计划项目(2006Z05-003-01)
四川省重大基础研究项目(04JY029-006-1,05JY029-109)
四川省杰出青年学科带头人基金后续资助项目(05ZQ026-038)
四川省重点建设学科项目(SZD0418)
关键词
猪生殖与呼吸综合征病毒
人工感染仔猪
原位杂交
分布
porcine reproductive and respiratory syndromeporcine reproductive and respiratory syndrome virus
piglet infected experimentally
in situ hybridization
distribution
