摘要
采用噻唑蓝(MTT)还原法、流式细胞术、光镜及透射电子显微镜技术等检测了梯度浓度的JA1(沙冬青提取物)对体外培养的小鼠肝癌细胞株Hep增殖的影响及诱导Hep细胞凋亡的作用;并检测了JA1对小鼠皮下移植性Hep细胞的生长抑制及诱导凋亡作用。结果显示,JA1可显著抑制小鼠Hep细胞的增殖,且这种抑制有浓度和时间依赖性。流式细胞仪分析显示,JA1处理后的Hep检测样本中有明显的DNA低含量颗粒("亚G1期"峰),细胞周期各时相分布发生了改变,细胞在G1期被阻滞。同时,JA1对小鼠皮下移植性Hep细胞也有明显的抑制作用。JA1灌胃试验组瘤组织细胞DNA直方图有明显的凋亡峰,细胞在G1期也被阻滞,光镜和电镜下可见大量凋亡肿瘤细胞。
To investigate the inhibitory effect of the extractive of Ammopiptanthus mongolicus （JA1）on hepatocarcinoma cells and its possible mechanisms,the hepatocellular carcinoma cell line of mice（Hep）was used as target cell. The inhibitory effects of JA1 on Hep cells were assayed by MTT,apoptosis were measured by flow cytometry in vitro and the antitumor effect of JA1 was also observed in Hep-bearing mice by flow cytometry assay, light microscopy and transmission electronic microscopy. JA1 had significant antiproliferative effects on Hep cells with dependence of time and dosage. In addition,JA1 not only could inhibit significantly the proliferation of Hep cells in vitro but also inhibit the growth of Hep hepatocarcinoma in vivo. Flow cytometric analysis indicated that JA1 could induce apoptosis on Hep cells in Hep-bearing mice and the G1 phase cell percentage was incrassation and the S phase cell percentage decrease. Morphological change of Hep cells undergoing apoptosis were also detected by light and electron microscopies. These result showed that JA1 had good inhibitory effects on Hep in vitro and in vivo and the mechanism was related to its apoptosis inducing activity.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2008年第4期332-337,共6页
Chinese Veterinary Science
基金
国家科技攻关计划项目(2001BA901A33)
关键词
沙冬青提取物
小鼠
Hep细胞
凋亡
JA1 （the extractive of Ammopiptanthus mongolicus）
mice
hepatocellular carcinoma cell line of mice（Hep）
apoptosis
