摘要
目的建立可供实验研究的稳定的兔VX2肝癌模型。方法新西兰大白兔59只,取瘤块(1mm3大小)开腹包埋法接种到兔肝脏左叶,于种植后第1,1.5,2,2.5,3,3.5,4周分别行超声学检查主要观察兔肝VX2成瘤检出情况。成瘤情况均经病理学证实。结果①围手术期3只兔因麻醉死亡,1只因感染死亡,成活率为93.2%(55/59);②兔肝原位肝实质内种植成功50个,其中伴有16只异位种植,单纯异位种植为3只,未发现病灶2只,原位成瘤率为90.9%(50/55);③接种后1周、1.5周经超声检查22例,发现病灶者8例,检出率为36.4%(8/22);其余肿瘤在2周及2周以后超声检查全部检出。两个时间段检出率有明显差异;④兔肝VX2肝癌的超声主要表现为略高回声、等回声、低回声,周围可见声晕。结论建立兔VX2肝癌模型成功率高、方法简单、周期短、动物死亡率低,种植2周后超声学筛选及监测本瘤有意义。
Objective To evaluate the feasibility of bulding VX2 liver tumor model in rabbit for medical experimental and clinical study. Methods Fifty-nine New Zealand white rabbits were embedded with VX2 tumor tissue （ about 1mm^3） in the lobe of the liver using laparotomy. Ultrasound examinations were performed at 1 th, 1.5th,2th,2.5th, 3th,3.5th,4th week after implantation respectively. The observations of Uhrasound examination were included the successful rate of implanting tumor and the image features of tumor. All the tumors were verified by histopathology. Results （1）The survival rate of the rabbits during perioperative period was 93.2% （ 55/59 ） ,3 cases died of anesthetic accident and 1 case died of infection;（2）Tumors were successfully implanted in 50/55（90.9% ） and 16 cases had ectopia implantation among them,3 cases were only with ectopia tumors and 2 cases without neoplasma;（3） Eight of 22 cases were deletted by ultrasound in 1.5 weeks ＇after implanting,and the rest tumors were detected by ultrasound in 2 weeks or beyond 2 weeks after implantation. The differences of ultrasound detection rate between the two period were obvious ;（4）Under the ultrasound, the tumors were hyperechoic, isoehoic or hyoechoic, eompanied with surrounding echo halo. Conclusion It is easy to establish the metastatic rabbit VX2 liver tumor model which can be duplicated conveniently. Its growth cycle and success rate of transplanting are satisfactory. To detect and screen the tumor in the experimental rabbits by ultrasound should be performed at before and after 2 weeks after implantation.
出处
《潍坊医学院学报》
2008年第1期1-4,I0001,共5页
Acta Academiae Medicinae Weifang
基金
山东省教育厅重点项目资助课题(J04E01)
山东省科技发展计划资助课题(200FGG30002032)
