摘要
目的:研究不同浓度As2O3对肾癌细胞株789-0的凋亡影响及联用IFN-α有无协同作用,并探讨其作用机制。方法:采用MTT法检测As2O3单用及联用IFN-α对细胞增殖的影响,流式细胞仪检测其对细胞周期和凋亡的影响。结果:(0.5-4)μmol/L的As2O3对肾癌789-0细胞的增殖均有一定抑制作用,且随浓度增加呈增强趋势。联用1000IU的IFN-α后,肾癌细胞的增殖抑制率明显高于相应浓度的单用As2O3组,具协同作用。2.83μmol/L(IC50)的As2O3作用于肾癌789-0细胞48h后呈现G2/M期阻滞,凋亡特征明显;联用IFN-α后呈现G0/G1期、G2/M期阻滞,凋亡率显著增高。结论:As2O3能抑制肾癌789-0细胞的增殖并诱导细胞凋亡,联用IFN-α可产生协同作用。其机制可能与干扰细胞周期的不同时相有关。
Objective： To study the apoptosis effect of different concentration arsenic trioxide（ As2O3 ） on renal carcinoma cell line 789 -0, and the synergistic effect of combination with IFN-α, as well as to probe its mechanism. Methods： MTT assay was used to test the effects of As2O3 alone and combination with IFN-α on cell proliferation of 789 -0. Cell cycle phase distribution and apoptosis of 789 -0 cell were measured by flow cytometry （FCM）. Results： The concentration of As2O3 within 0.5 -4μmol/L can inhibit the proliferation of 789 -0 cell to some extent, increasing with the dosage. The inhibition rate on cell proliferation increased more significantly by combination with 1000IU IFN-α than by the same concentration As2O3 alone, showing the synergistic effect. 48 hours after 2.83μmol/L As2Os （IC50） was applied to 789 -0 cell, G2/M phase was blocked, and the feature of apoptosis was notable. After the combination with IFN-α, Go/Gl phase and G2/M phase were both blocked , the apoptosis rate increased significantly. Conclusion： As2O3 can inhibit renal carcinoma cell line 789-0 from proliferation, and induce apoptosis. The combination of As2O3 and IFN-α has a synergistic effect. This may be related to the interference on the different phase of the cell cycle.
出处
《现代肿瘤医学》
CAS
2008年第5期718-720,共3页
Journal of Modern Oncology
