摘要
目的:检测胃癌组织和血清中VEGF、组织的p53、MVD的表达,并观察其与胃癌生物学行为之间的关系,以及三者的相关性。方法:应用酶联免疫技术(ABC-ELISA方法)检测73例胃癌患者术前血清和20例健康体检者血清中的VEGF表达水平,同时应用免疫组织化学染色方法检测癌组织和癌旁组织中VEGF、P53和CD34的表达。结果:胃癌患者术前血清VEGF表达水平明显高于健康体检者(VEGF:101.8±53.3ng/Lvs16.1±22.5ng/L,P<0.05)。胃癌患者术前血清VEGF表达水平随浸润深度、TNM分期、淋巴结转移、远处转移而增高(P<0.05),而与年龄、性别及病理类型无关。胃癌组织VEGF的阳性表达率为71.2%,癌旁组织中VEGF未见阳性表达,P<0.05;胃癌组织VEGF表达水平与胃癌浸润深度、TNM分期、淋巴结转移、远处转移有关(P<0.05),而与年龄、性别及病理类型无关。胃癌患者术前血清VEGF的表达水平与组织VEGF的表达呈正相关(r=0.346,P<0.01)。胃癌组织中p53的阳性表达率明显高于癌旁组织,P<0.05。p53在胃癌组织中的表达与性别、年龄、病理类型、浸润深度无关(P>0.05),与淋巴结转移、TNM分期、远处转移有关,P<0.05。MVD值在胃癌组织中明显高于胃癌癌旁组织,并与性别、年龄、病理类型无关(P>0.05),与浸润深度、淋巴结转移、TNM分期、无远处转移有关(P<0.05)。胃癌患者术前血清与组织VEGF表达水平分别与p53的表达水平呈正相关(r=0.316,P<0.01;r=0.290,P<0.05);胃癌患者术前血清与组织VEGF表达水平分别与MVD的表达水平呈正相关(r=0.434,P<0.01;r=0.512,P<0.01);胃癌患者p53与MVD的表达水平呈正相关(r=0.431,P<0.01)。结论:胃癌患者VEGF、p53与MVD胃癌的发生、发展、转移及预后起着重要的作用,有望成为胃癌术前诊断、判断转移危险的新的肿瘤标志物。
Objective： To investigate the expression and correlation of VEGF, p53 and MVD in human gastric cancer and discuss their significance in carcinogenesis, progression, invasion and matestasis of gastric cancer , as well as their relation with biological behaviour of gastric cancer. Methods：The expression of VEGF was evaluated in the serum samples of 73 preoperation gastric cancer patients as well as 20 serum samples of healthy persons using ABC - ELISA. The expression of VEGF , p53 and CD34 were studieded in the tissues of gastric cancer and normal tissue near cancer using immunohistochemistry. Results：The expression levels of VEGF in serum of preoperation gastric cancer patients were significantly higher than those in healthy persons（ VEGF： 101.8 ± 53.3 ng/L vs 16. 1 ± 22.5 ng/L,P 〈 0. 05 ）. The expression levels of VEGF in serum of preoperation gastric cancer patients were positively ralated to invasive depth ,TNM stage, metastasis of lymph node and distant metastasis（P 〈 0.05 ）. but not with age, sex and pathological type. The expession rate of VEGF in gastric cancer tissues was 71.2% ,while was 0 in the tissues near gastric cancer. There was significant difference between two groups（P 〈0.05）. The expression of VEGF in gastric cancer tissues was correlated with invasive depth ,TNM stage, metastasis of lymph node and distant metastasis （ P 〈 0.05 ）. but not with age, sex and pathological type. The expression levels of VEGF in serum of preoperation gastric cancer patients were positively correlated with that in gastric cancer tissues（ r = 0. 346,P 〈 0.01 ）. The expession of p53 in gastric cancer tissues was significantly higher than that in normal tissues near gastric cancer（ P 〈 0.05 ）. The expression of p53 in gastric cancer tissues was significantly correlated with the metastasis of lymph node, TNM stage and distant metastasis （ P 〈 0.05 ）. but not with invasive depth, age, sex and pathological type （ P 〉 0.05 ）. 3 The MVD in gastric cancer tissues was significantly higher than that in normal tissues near gastric cancer. The MVD in gastric cancer tissues was significantly correlated with the invasive depth, metastasis of lymph node, TNM stage and distant metastasis（P 〈 0.05 ）. but not correlated with age, sex and pathological type（ P 〉 0.05 ）. The expression of VEGF in serum of preoperation gastric cancer patients and in gastric cancer tissues was positively correlated with the expession of p53 respectively （ r = 0.316, P 〈 0.01 ; r = 0.290, P 〈 0.05 ）. The expression of VEGF in serum of preoperation gastric cancer patients and in gastric cancer tissues was positively correlated with the MVD respectively too （r=0.434, P〈0.01 ;r=0. 512,P〈0.01）. The expession of p53 was positively correlated with the MVD （r= 0.431, P 〈 0.01 ）. Conclusion.. VEGF, p53 and MVD might play important roles in the development, progression, metastasis and prognosis of human gastric cancer. They may be new markers for diagnosis and prognosis of gastric cancer.
出处
《现代肿瘤医学》
CAS
2008年第5期760-764,共5页
Journal of Modern Oncology
基金
哈尔滨市科技攻关计划项目(编号:2002AA9CS151-3)
