摘要
为获得能适用于普通PCR反应的橡胶基因组DNA,以橡胶树叶片为材料,分别采取CTAB法、SDS法及盐析法提取基因组DNA。并通过琼脂糖凝胶电泳、限制性内切酶酶切对3种方法提取的DNA样品进行检测。将它们在DNA产量、质量等方面进行比较,结果表明CTAB法DNA提取率高于SDS法和盐析法;由CTAB法提取的橡胶树基因组DNA能完全酶切,能满足PCR等后续分子生物学研究的需要。
In order to get the genomic DNA of Hevea brasiliensis which is suitable for normal PCR reaction, Leaves of rubber tree were used to isolated the genomic DNA by CTAB method,SDS method and salting out method. The Genomic DNA samples extracted by three methods were tested by agarose gel elctrophresis and restriction endonucleas digestion, Based on the comparative analysis of yield and quality of the DNA samples by three methods, the result showed that DNA yield of CTAB method was higher than that of SDS method and salting out method, the rubber tree genomic DNA isolated by the CTAB methods can not only be digested susceptibly by restriction endonucleas,but also can meet a demand for study of subsequent molecular research such as PCR analysis and so on.
出处
《安徽农学通报》
2008年第7期53-55,182,共4页
Anhui Agricultural Science Bulletin
基金
农业部热带作物栽培生理学重点实验室开放课题基金"橡胶死皮关键基因的克隆与功能鉴定"(KLOF0502)
