摘要
目的建立用自制兔抗人基质金属蛋白酶-11(matrix metalloproteinase-11,MMP-11)多克隆抗体鉴定月经血的免疫组化方法,探讨MMP-11蛋白酶的细胞定位。方法运用链霉卵白素-碱性磷酸酶(SAP)免疫组化法检测人月经血及外周静脉血涂片、阴道液涂片、子宫内膜石蜡切片和陈旧月经血痕涂片中的MMP-11。结果MMP-11存在于子宫内膜上皮细胞和间质细胞内;外周静脉血和阴道液涂片未见有MMP-11染色;陈旧月经血痕涂片罕见完整子宫内膜细胞,但能检测到微弱特异信号。结论用自制抗MMP-11多克隆抗体采用免疫组化技术能有效地检测MMP-11,区分月经血和静脉血。
Objective To prove the feasibility of detecting menstrual blood as well as its cellular localization with rabbit-anti-human matrix metalloproteinase-11（MMP-11） polyclonal antibody. Methods MMP-11 in menstrual blood, peripheral blood, vaginal liquid, aged menstrual bloodstain, and endometrium sections were assayed with SAP immunohistochemistry. Results MMP-11 was found only in menstrual samples within stroma and epithelium cells. Conclusion MMP-11 polyclonal antibody may be applied in the distinction between menstrual blood and venous blood.
出处
《法医学杂志》
CAS
CSCD
2008年第1期32-33,F0003,共3页
Journal of Forensic Medicine
基金
国家自然科学基金资助项目(30471933)
