摘要
目的通过检测银屑病患者皮损处上皮钙粘素、β-连环蛋白和细胞周期素D1的表达,探讨其在银屑病角质形成细胞高度增殖中的意义。方法用免疫组化方法检测正常组织及进行期银屑病皮损处上皮钙粘素、β-连环蛋白和细胞周期素D1蛋白的表达。结果银屑病患者皮损增生的表皮细胞上皮钙粘素和β-连环蛋白在颗粒层和基底细胞层表达明显降低,与正常人相比有显著性差异(P<0.01)。而细胞周期素D1在银屑病患者皮损基底细胞层表达显著升高,并与β-连环蛋白的异常表达相关。结论上皮钙粘素和β-连环蛋白表达的下调可能与银屑病角质形成细胞高度增殖有关,Wnt信号转导系统异常可能参与银屑病的发病机制。
Objective To examine the expressions of E-cadherin, β-catenin and cyclin D1 in the skin lesions of patients with psoriasis vulgaris, and understand their possible roles in keratinocyte hyperproliferation in these patients. Methods Immunohistochemistry was performed to detect the expressions of E-cadherin, β-catenin and cyclin D1 in the normal skin tissues and psoriatic lesions. Results In normal skin tissues, positive staining for E-cadherin and I^-catenin was detected in all layers of the normal epidermis at the sites of cell-cell junctions, and downregulation of E-cadherin and β-catenin expression was found in the granular layer and basal layer of the psoriatic lesions. Cyclin D1 overexpression was observed mainly in the basal layer of the lesions, which was correlated to abnormal expression of β-catenin. Conclusion Downregulation of E-cadherin and β-catenin expression and cyclin D1 overexpression in psoriatic skin are probably involved in keratinocyte hyperproliferation in psoriasis vulgaris.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2008年第4期545-547,共3页
Journal of Southern Medical University
基金
国家自然科学基金(30371295)~~
