摘要
目的检测哮喘患儿不同病期的白细胞介素12(IL-12)、转化生长因子β1(TGFβ1)与免疫球蛋白E(IgE)水平变化的规律,并探讨不同病期IL-12,TGFβ1水平与IgE水平的相关性,据此阐述它们在哮喘中的临床意义。方法采用ELISA方法检测85例哮喘患儿及30例正常儿童的血清IL-12,TGFβ1与总IgE水平。结果哮喘组血清IL-12,TGFβ1水平明显低于对照组,而IgE水平则哮喘组明显高于对照组,且发作期IL-12,TGFβ1水平(28.42±10.73ng/L,40.25±11.73pg/mL)明显低于缓解期(40.42±15.26ng/L,65.41±22.38pg/mL),差异有显著性(P<0.01),缓解期血清IL-12,TGFβ1水平明显低于对照组(67.42±20.58ng/L,178.54±90.56pg/mL),差异有显著性(P<0.01),发作期血清IgE水平(280.35±80.54IU/mL)明显高于缓解期(145.67±51.25IU/mL),差异有显著性(P<0.01),缓解期血清IgE水平明显高于对照组(53.61±13.32IU/mL),差异有显著性(P<0.01),哮喘患儿血清IL-12,TGFβ1水平与IgE呈负相关(P<0.01)。结论哮喘患儿存在IL-12,TGFβ1及IgE水平失衡,提示IL-12,TGFβ1在哮喘的发病中起着重要作用,检测它们的水平可为哮喘的诊断及判断病情提供依据。
Objective This study examined the changes of serum levels of interleukin 12 （ IL-12）, transforming growth factor β1 （TGFβ1） and immunoglobulin E （IgE） in children with asthma as well as the correlation of IL-12 and TGFβ1 with IgE in order to investigate their roles in asthma. Methods Serum levels of IL-12 , TGFβ1 and IgE were detected using ELISA in 85 asthmatic children at the acute and the remission stages. Thirty healthy children served as control group. Results Compared with the control group, serum IL-12 and TGFβ1 levels were significantly lower and serum IgE levels were significantly higher in the asthmatic group through the acute to the remission stages. Serum IL-12 and TGFβ1 levels （40.42 ± 15.26 ng/L and 65.41 ±22.38 pg/mL） significantly increased in the asthmatic group at the remission stage compared with those at the acute stage （28.42 ± 10.73 ng/L and 40.25 ± 11.73 pg/mL） （ P 〈 0.01 ）, but remained lower levels than those in the control group （67.42 ± 20.58 ng/L and 178.54 ± 90.56 pg/mL） （P 〈 0.01 ）. The asthmatic patients at the remission stage showed significantly decreased serum IgE levels （ 145.67 ±51.25 IU/mL） compared with those at the acute stage （280.35 ±80.54 IU/mL） （P 〈0.01 ）, but the IgE level in the remission stage was obviously higher than in the control group （53.61 ± 13.32 IU/mL） （P 〈 0.01 ）. Serum IL-12 and TGFβ1 levels were negatively correlated with serum IgE level in asthmatic children. Conclusions There might be an imbalance in serum IL- 12, TGFβ1 and IgE levels in asthmatic children. IL-12, TGFβ1 and IgE may play an important role in the pathogenesis of asthma. They may be useful in the diagnosis and severity evaluation of asthma.
出处
《中国当代儿科杂志》
CAS
CSCD
2008年第2期146-148,共3页
Chinese Journal of Contemporary Pediatrics
