摘要
用X射线照射离体EL-4淋巴瘤细胞,其诱导剂量(D1)为25～200mGy(剂量率12.5mGy/min),攻击剂量(D2)为1.5Gy(剂量率287mGy/min),D1和D2间隔6h。通过流式细胞仪检测其细胞凋亡百分数及其相关基因蛋白表达水平。其结果显示,当D1为25～100mGy,D2为1.5Gy;或D1为75mGy,D2为0.5～2.0Gy,与D2组比较,D1+D2组细胞凋亡百分数有显著意义降低。当D1为25～100mGy,D2为1.5Gy,与D2组比较,D1+D2组Bcl-2蛋白阳性百分数不同程度增加,Bax蛋白有显著意义降低,Bcl-2/Bax比值有显著意义增高;p53蛋白不同程度降低。其结果提示,在D1为25～100mGy,D2为0.5～2.0Gy,可诱导EL-4淋巴瘤细胞凋亡的适应性反应,其相关凋亡基因蛋白Bcl-2、Bax和p53发生的改变,与其细胞凋亡明显相关,可能在其适应性反应的机制中起到重要的调节作用。
EL-4 lymphoma cells were irradiated with the inductive doses （DI： 25 ～ 200 mGy, dose rate： 12.5mGy/min） and the challenging dose （D2：0.5 ～3.0 Gy, dose rate： 287 mGy/min）, and the time interval between D1 and D2 was 6 h. The percentage of cell apoptosis and the expressive levels of cell apoptosis-associated gene proteins were measured with flow cytometry. The percentages of cell apoptosis in the D1 ＋ D2 group with 25 ～ 100 mGy （D1） and 1.5 Gy （D2） or 75 mGy （D1） and 25 ～ 200 mGy （D2） were significantly lower than those in the D2 group. As compared with the D2 group, the positive percentage of cell Bcl-2 protein expression increased somewhat, and Bax decreased significantly, meanwhile the ratio of Bcl-2/Bax increased significantly and p53 decreased somewhat in D1 ＋ D2 group with 25 ～ 100 mGy （D1） and 1.5 Gy （D2）. The adaptive response of EL-4 lymphoma cell apoptosis could be induced by pre-irradiation with 25～ 100 mGy. Meantime, the expressive levels of cell apoptosis-associated gene Bcl-2, Bax and p53 proteins could change accordingly with cell apoptosis. These gene protein changes may play an important role in the mechanism of the adaptive response.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
2008年第2期70-73,共4页
Journal of Radiation Research and Radiation Processing
基金
国家自然科学基金(30570546)资助
关键词
电离辐射
淋巴瘤细胞
细胞凋亡
凋亡基因
抗凋亡基因
适应性反应
Ionizing radiation, Lymphoma cell, Cell apoptosis, Apoptotic gene, Anti-apoptotic gene, Adaptive resDonse
