摘要
对水稻内生固氮菌N1(短小芽孢杆菌,Bacillus pumilus)、N2(阴沟肠杆菌,Enterobacter cloacae)和N3(鹑鸡肠球菌,Enterococcus gallinarum)的一些生化特性及其对烟草和玉米的侵染进行了研究。采用三亲交配法将标记基因nifH-lacZ、nifHDK-lacZ和gfp分别导入到固氮菌中,用携带标记基因的固氮菌分别接种烟草云烟85和玉米吉甜6号,对感染固氮菌的烟草和玉米材料进行β-半乳糖苷酶组织化学染色和激光共聚焦扫描显微镜、透射电子显微镜和扫描电子显微镜观察。光学显微镜和电子显微镜观察结果表明在接菌的烟草和玉米部分根细胞中和细胞间隙都发现了固氮菌,在接菌的玉米茎的维管组织细胞中也发现了固氮菌,推测固氮菌从玉米根开始向上移动。接种固氮菌N3的玉米与对照相比,在鲜重上提高了18.53%。
Some biochemical properties of rice endophytic diazotrophic bacteria N1 （Bacillus pumilus）, N2 （ Enterobacter cloacae） and N3 （Enterococcus gallinarum） were characterized in this study. The plasmids harboring the reporter gene lacZ fused to the promoters of nifH and nifHDK , ＂and the reporter gene gfp as well, were fast transformed into rice endophyfic diazotrophic bacteria respectively through triparental mating. After the conjugated bacteria were inoculated into tobacco Yunyan 85 and maize Jitian 6, the staining of β-galaetosidase was carried out on the tobacco and maize roots and the observation was made under the confocal laser scanning microscope, transmission electron microscope and scanning electron microscope. The results showed that the rice endophytic diazotrophic bacteria were not only present in epidermal cells, cortex cells and intercellular spaces of tobacco and maize roots, but also found in vascular tissue cells of maize stem also, indicating that the bacteria had migrated from the roots into the stem. Data showed that the the fresh weight of maize plants inoculated with rice endophytic diazotrophic bacteria N3 was 18.52% more than that of the control .
出处
《云南植物研究》
CAS
CSCD
北大核心
2008年第2期211-220,共10页
Acta Botanica Yunnanica
基金
福建省科技厅重点科技项目(2004N026)
福建省自然基金项目(B0410009)
福建省教育厅科技项目(JB03125)资助
关键词
固氮菌
生化特性
烟草
玉米
侵染
Endophytic diazotrophic bacteria
Biochemical characteristics
Tobacco
Maize
Infection
