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缺血后处理对大鼠缺血再灌注心肌缝隙连接蛋白43的影响 被引量：5

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摘要 目的观察缺血后处理对缺血再灌注心肌缝隙连接蛋白43(Cx43)的影响,进一步探讨其减少再灌注心律失常的可能机制。方法将雄性Wistar大鼠随机分为假手术组、缺血再灌注组、缺血预处理组、缺血后处理组,每组8只。建立在体心肌缺血再灌注模型,观察心律失常的发生情况,应用免疫组织化学SP法检测Cx43在各组缺血再灌注心肌中的表达,应用反转录-聚合酶链反应(RT-PCR)法半定量检测各组Cx43的mRNA的表达水平。结果与假手术组比较,缺血再灌注组心律失常评分显著增高,心室肌Cx43含量显著降低、分布紊乱,Cx43的mRNA水平显著降低(P<0.01),与缺血再灌注组比较,缺血后处理组的心律失常评分显著降低,心室肌Cx43含量增高、分布规律,Cx43的mRNA水平显著增高(P<0.01)。结论缺血后处理能通过抑制Cx43蛋白降解和再分布、上调其mRNA水平起到保护Cx43蛋白的作用,进而减少再灌注心律失常的发生。
作者 李永慧 王电烨 李胜建
机构地区 山西医科大学 山西中医学院附属医院 山西医科大学第二医院
出处 《中西医结合心脑血管病杂志》 2008年第4期418-420,共3页 Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
关键词 缝隙连接蛋白43 心律失常 缺血预处理 缺血后处理 缺血再灌注损伤
分类号 R541 [医药卫生—心血管疾病]
  • 相关文献

参考文献9

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二级参考文献12

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共引文献12

同被引文献78

引证文献5

二级引证文献88

中西医结合心脑血管病杂志
2008年 第4期

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