摘要
通过对某炼油厂生化反应池的活性污泥的富集与驯化,得到一组能以芘为唯一碳源的混合微生物DP.将混和菌DP分离纯化,可得到6种芘降解菌DP1,DP2,DP3,DP4,DP5和DP6.通过显微镜观察各降解菌的菌落特征及革兰氏染色实验,初步判断DP1为细菌,其他为放线菌.在芘质量浓度为100 mg.L-1的培养基中培养44 h后,6种菌对芘的降解率分别为46.2%,83.2%,23.6%,11.3%,53.3%,13.6%,其中DP2为芘的高效降解菌.
A microbial consortium named DP was cultivated from the refinery activated sludge with pyrene as the single carbon and energey source. Six pure strains were isolated and purified from DP,which were named as DP1 ,DP2, DP3, DP4 ,DP5 and DP6. DP1 was a specy of bacteria and the others were species of actinomycetes according to morphological observations, including colony observation and light microscope examination basing on the Gram staining of the strains. To measure their degradation capability, the removal efficiencies of pyrene were 46.2 %, 83.2%, 23.6 %, 11.3 %, 53.3% and 13.6% respectively after 44 hours in the mineral salts medium under initial pyrene concentration of 100 mg · L^-1. DP2 was a kind of high-efficient degradating strain towards pyrene.
出处
《华侨大学学报（自然科学版）》
CAS
北大核心
2008年第2期267-269,共3页
Journal of Huaqiao University(Natural Science)
基金
福建省自然科学基金资助项目(D0710019)
国务院侨办科研基金资助项目(06QZR09)
关键词
芘
降解菌
分离
纯化
生物降解
pyrene
degradating strains
isolation
purification
microbiological degradation
