摘要
目的比较观察青年猫(1-3岁)和老年猫(12-13岁)小脑皮质浦肯野细胞(Purkinje cell,PC)的超微结构,探讨其年龄相关变化的生理意义。方法常规电镜包埋、切片、染色,透射电镜下观察并拍照。结果老年组PC粗面内质网碎裂,核糖体解离;线粒体膨解,嵴减少;高尔基复合体扁平囊扩张,极性退化;脂褐素聚积;膜性髓样结构与空泡变性出现;核膜内陷,染色质固缩;生物膜系统结构破坏等。结论衰老过程中细胞器结构退化可能影响老年PC物质合成、能量供应、信息传递及神经元的结构稳定,导致衰老PC死亡,推测与老年个体运动调节和运动学习等小脑功能退化有重要联系。
Objective To comparatively observe the ultrastructural changes of Purkinje cells （PCs） in the cerebellar cortex of young adult cats （1-3 years） and old cats （12-13 years）, and to explore the possible physiological impacts of these age-related changes. Methods After routine microscopical embedding, sectioning, and staining, the ultrastructures of the PCs were detected under transmission electron microscopy and photos were taken. Results Compared with the young cats, the old cats exhibited the following changes： the rough endoplasmic reticulum （rER） collapsed, accompanied with desquamation of ribosomes; the mitochondria swelled, with decomposed cristae; the sacs of the Golgi apparatus showed dilatation and abnormal polarities; the lipofuscin accumulated heavely in the perikaryon; the myelin-like structures and the vacuolations emerged; the karyotheca invaginated; the chromatin condensed, and the biomembrane was destroyed. Conclusion Age-related degeneration of the organelles in the PCs might have important impacts on the functional reduction of substance synthesis, energy provision, information transmission, and cytoskeletal stablization during aging, which might lead to PC＇s death. We speculate that those ultra structural alterations in aging PCs might have significant relationships with cerebellar dysfunctions, such as retrogression of motor control and motor learning, in senescent individuals.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2008年第2期191-195,共5页
Chinese Journal of Histochemistry and Cytochemistry
基金
安徽省教育厅科研基金(KJ2007B330zc)
安庆师范学院科研启动金(K050000000126
044-K05000000075)
安徽省重点实验室科研基金资助课题
