摘要
目的:探讨针刺治疗脂肪肝的机制。方法:33只雄性SD大鼠随机分为正常对照组(11只)、模型组(12只)和电针治疗组(10只)。以高脂饲料饲养8周建立非酒精性脂肪肝(NAFLD)大鼠模型,8周末,处死其中3只(正常对照组1只,NAFLD组2只),以验证造模成功。电针治疗组施以电针治疗,取"足三里""丰隆""三阴交""太冲"穴(疏密波,频率2 Hz,强度4 mA),每次15 min,连续治疗4周。12周末处死所有动物,用比色法检测血糖、血清游离脂肪酸含量,用放射免疫法测定血清胰岛素含量,计算稳态模式胰岛素抵抗指数、肝指数及HE染色观察大鼠肝组织病理学改变。结果:与正常对照组比较,NAFLD组大鼠的血糖、血清胰岛素、血清游离脂肪酸、胰岛素抵抗指数、肝指数指标均升高(P<0.05),肝脏中出现中重度弥漫性脂肪变性,尚可见肝细胞水肿等现象;电针治疗组上述5项指标均显著低于NAFLD组(P<0.05),其肝脂肪变性程度亦显著减轻。结论:针刺治疗可以通过改善胰岛素抵抗状态治疗NAFLD。
Objective To investigate the mechanism of electroacupuncture （EA） in treating nonalcoholic fatty liver disease （NAFLD） in rats. Methods Thirty male SD rats were randomized into normal control, NAFLD model and EA groups, with 10 cases in each. NAFLD model was established by feeding the rats with high-fat food for 8 weeks. EA （2 Hz, 4 mA） was applied to ＂Zusanli＂ （ST 36）, ＂Fenglong＂ （ST 40）, ＂Sanyinjiao＂ （SP 6）, and ＂Taichong＂ （LR 3） for 15 min, once daily for 4 weeks. Fasting blood sugar （FBG） and serum free fatty acids （FFA） were detected with chromatometry; serum insulin （INS） was determined with radioimmunoassay; homeostasis model assessment insulin resistance （HOMA-IR） and liver index （LI） were calculated. Liver tissue was cut into sections for detecting pathological changes. Results Compared with normal control group, FBG, INS, FFA, HOMA-IR and LI of model group increased significantly （P〈0.05）, and hepatic diffuse steatosis with cellular necrosis and inflammatory cell infiltration was obvious. In comparison with model group, all the 5 indexes in EA group decreased significantly （P〈0.05）, and pathological changes as hepatic steatosis, etc. were significantly reduced. Conclusion EA may be used to effectively improve NAFLD by lowering insulin resistance.
出处
《针刺研究》
CAS
CSCD
2008年第2期111-115,共5页
Acupuncture Research
关键词
非酒精性脂肪肝
胰岛素抵抗
针刺
Nonalcoholic fatty liver
Insulin resistance
Electroacupuncture
