摘要
目的:本实验研究三氧化二砷(As_2O_3)对人类腺样囊性癌ACC-2细胞体外增殖及其表达VEGF和bFGF的影响。探讨As_2O_3对人类腺样囊性癌VEGF和bFGF基因的作用机制。方法:采用MTT法检测不同浓度和作用时间下As_2O_3对ACC-2的增殖/抑制效应.倒置显微镜下观察腺样囊性癌ACC-2细胞生长情况;以RT-PCR、Western blotting方法检测As_2O_3作用后腺样囊性癌ACC-2细胞的血管生成相关因子VEGF和bFGF表达的变化。结果:As_2O_3作用48h内对ACC-2细胞有促进生长作用,48、72h对细胞有明显抑制,并呈时间一剂量依赖关系。RT-PCR检测显示,As_2O_3作用后腺样囊性癌ACC-2细胞VEGF和bFGF基因表达无明显变化。Western blotting检测显示,腺样囊性癌ACC-2细胞VEGF和bFGF蛋白表达与As_2O_3药物浓度和作用时间呈负相关。结论:(1)As_2O_3体外作用人类腺样囊性癌ACC-2细胞后48h内有促生长作用,48h后有明显抑制作用;(2)As_2O_3可明显抑制腺样囊性癌ACC-2细胞血管生成相关因子VEGF和bFGF基因蛋白表达,可能具有抗腺样囊性癌血管形成的作用。
Objective： To investigate the effect of As2O3 on salivary adenoid cystic careinoma-2（ACC-2） cells proliferation in vitro and expression of VEGF and bFGF of the cells. Methods： ACC-2 cells were cultured with As2O3 in different concentrations （0.2.4.6 μmol/L）for different time （24 h.48 h ,72 h）. Cytomorphology was observed by phasecontrast microscopy. As2O3 cytotoxity was deter-mined by MTT assay. Gene and protein expression of VEGF and bFGF were investigated by RT-PCR and Western blotting analysis respectively. Results： Proliferation of ACC-2 cells were markedly promoted 24h after As2O3 administration. However, As2O3 induced a dose- and time-dependent tumor eell proliferation. The results of RT-PCR revealed no significant difference between gene expression of VEGF and bFGF. A negative relationship between As2O3 concentration and protein product of these two cytokines were observed. Conclusion： （ 1 ） ACC- 2 cells might be markedly depressed after 48 h by As203 administration in a time- and dose-dependenty reliable pattern. In contrast, the cells＇ proliferation could be promoted within 48 h. （2）As2O3 toxicity can decrease protein product of VEGF and bFGF apparently. So might depress vessel formation of ACC-2 cells.
出处
《口腔颌面外科杂志》
CAS
2008年第2期87-91,共5页
Journal of Oral and Maxillofacial Surgery
