摘要
目的:研究不同剂量1318 nm Nd:YAG激光的生物学效应。方法:用波长为1318 nm的Nd:YAG激光连续照射体外培养的处于对数生长期的人胰腺癌PC-3细胞。在低剂量组内分组分别照射0 s、2 s、4 s、6 s、8 s、10 s、12 s、14 s,每天1次,连续照射3次;在高剂量组内分组分别照射0 s、10 s、15 s、20 s、25 s、35 s、50 s,照射1次。照射结束后,继续培养24 h,噻唑蓝比色法(MTT)测定各组OD值,研究细胞增殖情况。结果:在低剂量范围内,随着激光照射剂量的提高,PC-3细胞的OD值逐渐升高(P<0.05)。而在高剂量范围内,随着激光照射剂量的提高,PC-3细胞的OD值逐渐降低(P<0.05)。结论:1 318 nm Nd:YAG激光,在低剂量范围内照射PC-3细胞,具有明显促进其增值的作用,呈剂量依赖性,在临床应用上应避免该副作用的发生;而在高剂量范围内照射PC-3细胞,具有明显抑制其增殖的作用,呈剂量依赖性,对于指导临床应用具有重要价值。
Objective：To investigate the biological effect of varing intensities of 1 318 nm Nd ： YAG laser on human pancreatic carcinoma cells （PC-3）. Methods： Eight groups of PC-3 cells were cultured in 96-well plates for 12 h and then irradiated with low intensity 1 318nm Nd：YAG continuous wave laser for different time intervals（0 s ,2 s ,4 s ,6 s, 8 s,10 s,12 s ,14 s）. The laser power was 1.7 W and spot diameter 5 mm （laser dose 0- 121.27 J/cm^2）. Cells were irradiated once a day and overall 3 days and cultured for the next 24 h. Seven groups of PC-3 cells were cultured in 96- well plates for 12 h and then irradiated with high intensity 1 318 nm Nd：YAG continuous wave laser for different time intervals（0 s,10 s,15 s,20 s,25 s,35 s,50 s）. The laser power was 1.6 W and spot diameter 5 mm （laser dose 0 407.6 J/cm^2）. Cells were irradiated once and euhured for the next 24 h. MTF was used to assess the cell proliferation of the two groups, respectively. Results. A significant stimulative and inhibitive effect of low and high intensity 1 318 nm laser on PC-3 cell proliferation was found respectively （P 〈 0.05 ）. Conclusion： The PC-3 cell proliferation can be stimulated by low intensity 1 318 nm Nd. YAG continuous wave laser, which suggests that this low intensity laser could be potentially harmful to patients with pancreatic carcinoma. While it＇ s proliferation can be inhibited by high intensity 1 318 nm Nd：YAG continuous wave laser, which suggests that this high intensity laser could be beneficial to patients with pancreatic carcinoma.
出处
《激光生物学报》
CAS
CSCD
2008年第2期172-175,共4页
Acta Laser Biology Sinica
