摘要
研究了Nostoc sp.FACHB87、Nostoc paludosum FACHB89等10株念珠藻的甲醇提取物对酪氨酸酶的抑制作用。结果表明,随着提取物浓度的升高,对酪氨酸酶的抑制作用也增强。其中Nostoc sp.FACHB892的甲醇提取物对酪氨酸酶抑制作用最强,在33.3μg/mL的最高测试浓度下,抑制率可达到60.54%;Nostoc sp.FACHB95、Nostoc sp.FACHB106、N.punctiforme FACHB252、N.calcicola FACHB389和N.parmiloides FACHB392的甲醇提取物,在33.3μg/mL的最高测试浓度下,抑制率分别为41.55%、37.58%、46.94%、52.12%和47.95%。Nostoc sp.FACHB892的甲醇提取物对酪氨酸酶的抑制作用表现为可逆抑制,抑制类型为非竞争型抑制。
The inhibitory effects on tyrosinase of the methanolic extracts from 10 Nostoc strains were investigated. The results show that the inhibitory action for the above 6 strains of Nostoc enhanced with the increase of concentration of the methanolic extracts. The methanolic extracts of Nostoc sp. FACHB892 has great inhibitory capacity on tyrosinase, of which the inhibitory rate is 60. 54% at concentration of 33.3μg/mL, while the methanolic extracts of other 5 strains （Nostoc sp. FACHB95, Nostoc sp. FACHB106, N. punctiforme FACHB252, N. calcicola FACHB389 and N. parmiloides FACHB392） exhibitted weaker inhibitory effects. The inhibitory rates are 41.55% ,37.58% ,46.94% ,52. 12% and 47.95% respectively at the same concentration. The inhibitory mechanism of the methanolic extracts of Nostoc sp. FACHB892 on tyrosinase is reversible and noncompetitive indicated by the enzymatic kinetic analysis.
出处
《武汉植物学研究》
CSCD
北大核心
2008年第2期179-182,共4页
Journal of Wuhan Botanical Research
基金
国家自然科学基金资助项目(30671611)
