摘要
目的:观察健胃愈疡颗粒(柴胡,党参,白术,延胡索等)对幽门螺杆菌相关性胃炎胃黏膜乳癌相关肽PS2和血小板活化因子(platelet activating factor,PAF)表达的变化,并分析其可能的作用机制。方法:80例幽门螺杆菌感染慢性胃炎患者随机分为2组,治疗组42例给予健胃愈疡颗粒治疗,对照组38例给予西药三联药物治疗。应用胃镜下活检胃黏膜组织快速尿激酶和Warthin-Starry染色法检测幽门螺杆菌,并同时检测乳癌相关肽,血小板活化因子表达的变化和氨基己糖及磷脂含量。第4周复查胃镜。结果:治疗组总有效率高于对照组(P<0.05)。健胃愈疡颗粒治疗后磷脂和氨基己糖的含量显著高于治疗前(P<0.01)。健胃愈疡颗粒组氨基己糖,磷脂含量增高,与对照组比差异有显著性(P<0.01)。免疫组化,Western blotting检测显示:健胃愈疡颗粒可提高PS2和下调PAF的表达,而对照组对胃黏膜PS2和PAF的表达影响不大。结论:健胃愈疡颗粒抗胃炎的作用机理可能与杀灭Hp和影响胃黏膜的疏水性有关。
AIM： To observe the effect of Jianwei Yuyang Granule （Radix Bupleuri,Radix Codonopsis, Rhizoma Atractylodis macrocephalae,Rhizoma Corydalis, etc. ） on the expressions of PS2 and platelet activating factor （PAF） of helicobacterpylori-associated gastritis and study the potential mechanisim. METHODS： 80 cases were divided into two groups. Jianwei Yuyang Granule group （42 cases） was given Jianwei Yuyang Granule and the control group （38 cases） was given three kinds of western medicines combined. H. pylori was detected by urease test on biopsy specimen of gastric mucosa and Warthin-Starry method and the changes of expressions of PS2 and platelet activating factor （PAF） were observed. The contents of aninohexose and phosphatide in gastric mucosa were determined at the same time. All patients were rechecked gastroscope in the 6th week. RESULTS： The total effect rate of Jianwei Yuyang group was higher than that of the control group （ P 〈 0.05 ）. The contents of aninohexose and phosphatide after treatment in Jianwei Yuyang group were significantly higher than that before treatment. The Immunohisch- emical and Western blotting method showed that Jianwei Yuyang Granule could reduce the expression of PAF, raise the expression of PS2 , but there were not these kinds of action in the control group. CONCLUSION ： This implies that the possible anti-gastritis mechanisims is Jianwei Yuyang Granule kills Hp and influences the hydrophobicity of gastric mucosa.
出处
《中成药》
CAS
CSCD
北大核心
2008年第4期480-483,共4页
Chinese Traditional Patent Medicine
