摘要
在多年的小麦（Ｔｒｉｔｉｃｕｍ ａｅｓｔｉｖｕｍ Ｌ．）花药培养育种及对多培养基应用研究的基础上，从调整培养基的大量元素和微量元素入手，建立了癸培养基。经过Ｌ１６（４＾５）正交实验，筛选出ＮＨ４ＮＯ、ＫＮＯ３、ＭｇＳＯ４、ＭｎＳＯ４在培养基中较优的配比。在培养基对比实验中，癸培养基的愈伤组织平均诱导率分别比Ｃ１７、Ｗ１４、Ｍ６培养基提高了３０．３１％、５０．６６％和５７．９６％。
Gui culture medium has been developed by adjusting the concentration of macro- and micro- elements, based on the studies of various media applying to wheat ( Triticum aestivum L.) anther culture breeding for many years. An appropriate ration among NH4NO3, KNO3, MgSO4 and MnSO4 in the medium was selected by orthogonal design L16(45). For comparison of different media namely C17, W14 and N6 medium, the average frequencies of callus induction were increased by 30.31%, 50.66% and 57.96% respectively when Gui medium was used. And the Gui medium showed all its advantages in various crosses. The frequencies of wheat anther callus induction showed an increase of 54.25% to 64.07% and the callus development was also promoted when adding 0.9 mg/L rare earth addition to the Gui medium.
基金
"八五"国家生物技术攻关资助项目(编号 85-722-02-01-08)
关键词
小麦
花药培养
癸培养基
愈伤组织
诱导
Wheat, Anther culture, Gui medium, Callus induction, Rare earth
