摘要
目的:采用高效液相色谱法测定土茯苓中薯蓣皂苷元的含量。方法:色谱柱为Diamonisil(TM钻石)C18柱(150mm×4.6mm,5μm);以乙腈-水(90:10)为流动相;检测波长203nm;柱温35℃;流速:1.0mL.min-1。结果:薯蓣皂苷元进样量在0.4896μg^2.448μg范围内线性关系良好(r=0.9995);加样回收率为97.1%,RSD为1.23%。结论:方法操作简便,分离效果良好。
Objective：A HPLC method for determination of yam in Rhizoma smilacis glabrae was established. Method：The chromatographic conditions was listed below： Dianonisil ODS C18 column, UV detector at 203nm of wavelength, a mobile phase of acetonitrile-H2O（90：10）, and 1.0mL · min^-1 of flow rate. Result： The calibration Curves were linear for yam within 0.4896μg-2.448μg（r=0.9995）. The recovery was 97.1%（RSD=0.43%）. Conclusion ： This method is simple and the separate result is satisfactory.
出处
《辽宁中医药大学学报》
CAS
2008年第5期159-160,共2页
Journal of Liaoning University of Traditional Chinese Medicine
