摘要
目的研究尼美舒利对人胃癌敏感细胞株SGC7901及多药耐药细胞株SGC7901 VCR的影响,初步探讨其逆转人胃癌耐药的机制。方法MTT法测定细胞生长抑制率、流式细胞仪检测细胞内Rh123浓度和P-170、GST-π表达水平变化。结果尼美舒利对SGC7901及SGC7901 VCR细胞的生长抑制呈明显时间剂量依赖关系。但尼美舒利对SGC7901 VCR细胞效应强度明显弱于SGC7901细胞。尼美舒利能提高SGC7901 VCR细胞内Rh123荧光强度,下调P-170和GST-π的水平(P<0.05)。结论尼美舒利对人胃癌SGC7901 VCR耐药有一定逆转作用,作用机制可能与下调P-170和GST-π的水平有关。
Objective To evaluate the effect of nimesulide on gastric cancer cell line SGC7901VCR and SGC7901 and explore the mechanism of which reversing multi-drug resistance to gastric cancer cell line SGC7901VCR. Methods Cell suppression rates were detected by MTT assay. Rh123 concentration in cells and expressions of P-170, GST-π were analyzed by flow cytometry. Results Nimesulide inhibited the cell growth of SGC7901VCR and SGC7901 in a dose and time dependent manner, especially for SGC7901 cells. Rh123 fluorescence intensity in the SGC7901VCR cell was obviously increased and P- 170,GST-π expressions were decreased by nimesulide（ P 〈 0. 05 ）. Conclusion Nimesulide can partly reverse multi-drug resistance to gastric cancer cell line SGC7901VCR, the mechanism of which may be associated with decreasing expressions of P- 170 and GST-π.
出处
《医药导报》
CAS
2008年第5期515-517,共3页
Herald of Medicine
