摘要
目的:建立小鼠胆总管结扎致肝纤维化模型,并对其进行初步评价。方法:随机给予小鼠胆总管结扎和假手术,进行血清学指标(AST、ALT、ALP、TBIL、DBIL)检测;HE染色和天狼猩红染色进行形态学观察;采用免疫组化染色分析α-SMA和CK-19表达;实时荧光定量PCR检测α-SMA、Collagen-1 mRNA的表达。结果:小鼠胆总管结扎后2周可见CK-19阳性的棕褐色细胞明显增生,模型组血清AST、ALT、ALP、TBIL、DBIL明显增高,α-SMA、Collagen-1表达明显增强。结论:成功构建小鼠胆总管结扎致肝纤维化模型。
Objective：To establish and evaluate liver fibrosis model induced by bile duct ligation in mouse. Methods：The bile ducts of the mice were occluded by ligation. Serum AST,ALT,ALP,TBIL and DBIL were determined; the morphological observation was done by HE staining and Sirius red staining,expressions of α-SMA and CK-19 were analyzed by immunohistochemistry staining;mRNA expression of α-SMA and Collagen-1 in liver tissues were assayed by real-time PCR. A sham-operation group was used as a control. Results：Chocolate brown cells with positive expression of CK-19 and α-SMA significantly proliferated 2 weeks after bile duct ligation.Serum AST, ALT,ALP,TBIL and DBIL levels of model group were much higher than those of control group;mRNA expression of α-SMA and Collagen-1 in liver tissue by real-time PCR were increased significantly in model group. Conclusion：The mouse liver fibrosis model can be established successfully by bile duct ligation.
出处
《重庆医科大学学报》
CAS
CSCD
2008年第4期390-393,共4页
Journal of Chongqing Medical University
基金
国家自然科学基金(30670965)
教育部重点课题(207099)
关键词
胆总管结扎
肝纤维化
动物模型
Bile duct ligation
Liver fibrosis
Animal model
