摘要
目的探讨孕妇巨细胞病毒(HCMV)感染及其母婴垂直传播状况。方法应用酶联免疫吸附法(ELISA)筛查11859例孕妇HCMV的特异性抗体(IgG及IgM),同时应用荧光定量PCR(FQ-PCR)技术检测其中248例具有活动性HCMV感染孕妇的羊水或胎儿脐血或产后直接抽脐血检测HCMV-DNA。结果孕妇HCMV感染率为95.3%(11306/11859),孕妇活动性HCMV感染率为3.5%(417/11859),孕中期羊水的HCMV-DNA阳性率为12.1%(12/99);孕晚期脐血或产后直接抽脐血HCMV-DNA阳性率为6.0%(9/149),羊水的HCMV-DNA阳性率与分娩期脐血及及新生儿血相比较,差异无统计学意义(P>0.05)。结论孕产妇孕前绝大多数已感染过HCMV,测定孕中期羊水的HCMV-DNA,对早期诊断先天性HCMV感染具有重要意义。
Objective To determine the prevalence of human cytomegalovirns （HCMV） infection among pregnant women and assess its maternal - neonatal transmission. Methods ELISA method was used to screen HCMV specific antibody （ IgG ＆ IgM） of 11, 859 pregnant women, of whom 248 cases was diagnosed as active HCMV infection. The infected women were performed amniocentesis or cordocentesis with fluorescent quantitative PCR （FQ - PCR） assay, Results The HCMV infection rate among preg- nant women was 95.3 %, with an active HCMV infection rate of 3.5 %. The HCMV - DNA positive rate of amniotic fluid in mid - trimester was 12. 1%, while the HCMV - DNA positive rate of cord blood in late pregnancy or after delivery was 6. 0 %. There were no differences of HCMV - DNA infection rates among amniotic fluid, cord blood during delivery, or neonatal blood （ P 〉 0.05 ）. Conclusion Most of the pregnant women were infected by HCMV before pregnancy, and the HCMV maternal - neonatal transmission rate was higher in mid - trimester than in late - trimester, Therefore, the amniocentesis of HCMV - DNA in mid - trimester of pregnancy has important implications.
出处
《中国生育健康杂志》
2008年第2期91-94,共4页
Chinese Journal of Reproductive Health
基金
广东省医学科研基金立项课题(项目编号A2006087)
关键词
孕妇
婴儿
巨细胞病毒
感染
Pregnant women
Infant
Human cytomegalovirus
Infection
