摘要
木聚糖是植物中半纤维素的主要成分,含量仅次于纤维素的可再生多糖资源.木聚糖酶的使用可以有效提高半纤维素的利用,降低原料成本,减少污染,保护生态环境.应用快速有效的数学统计方法对木聚糖酶生产菌黑曲霉(Aspergillus niger)FS418液体发酵培养基进行优化.首先采用Plackett-Burman设计对培养基中8因素进行筛选,获得影响产酶3个最重要的营养组分:蛋白胨、麸皮、甘蔗渣;然后采用响应面分析法对3个因素进行3水平的优化,得到它们的最佳组合:麸皮6.0%、蛋白胨0.2%、甘蔗渣0.4%.优化后液体发酵水平比初始设计有了较大提高,最终产酶水平达到5147.29U/mL.实测值与模型的预测值只有0.8%的误差.
Xylan is the main component of hemicellulose from plants, one important repro- ducible polysaccharide resource. The application of xylanase can improve making use of hemicel- lulose, decrease material cost, reduce pollution and protest entironment. Statistical methods are used to determine the optimum fermentation culture medium for xylanase produced by Aspergillus niger FS418. A Plackett-Burman design is used to screen 3 important variables from 8 factors which affected xylanase fermentation. They are peptone, bagasse and bran in the culture medium. The optimum component obtain by Response Surface Methodology （RSM） is bran 6.0%, peptone 0.2%, bagasse 0.4% respectively. After the optimization, the yield of xylanase increased markedly compared with the initial and reached 5147.29U/mL, only 0.8% difference from the forecast by RSM.
出处
《生物数学学报》
CSCD
北大核心
2008年第1期143-150,共8页
Journal of Biomathematics
基金
福建省教育厅资助项目(JA05203)
