摘要
目的：建立超高效液相色谱-电喷雾串联四极杆质谱快速测定生活饮用水中莠去津、灭草松的方法。方法：水样经WatersOasisHLB固相萃取净化，应用超高效液相色谱／电喷雾串联四极杆质谱仪多离子反应监测（MRM）定量法检测莠去津、灭草松，建立了定量检测生活饮用水中莠去津、灭草松的快速、准确、高灵敏度的方法。结果：经方法学验证。该方法对莠去津、灭草松的最低检出限LOD分别为0．08、0．8μg／L（进样量10μl），最低定量限LOQ分别是0．1、1．0μg/L。在1．0～100．0μg／L的线性范围中，相关系数r〉0．9999，回收率：87．6％～103．0％。结论：方法灵敏度高、操作简单、定量准确、测定浓度范围宽阔，是环境水质样品中莠去津、灭草松含量检测的理想方法。
Objective： A UPLC - ESI - MS/MS method was developed for the determination of atrazine and bentazone in drinking water. Methods： For clean- up and concentration solid phase extraction （SPE） with OASIS HLB cartridges were used, or membrane filter was used to remove dirties. UPLC analysis was performed on a Waters ACQUITY UPLC BEH C18 1.0 × 50 mm column, water and acidified acetonitrile was used as mobile phase. The 2 agents were determined by UPLC - ESI - MS/MS and quantified with multiple reaction monitoring （MRM） mode. Results：The detection limits （LOD） for atrazine and bentazone were 0. 08,0. 8μg/L,respectively. The limits of quantification （LOQ） were 0. 1,1.0μg/L, respectively. The linear ranges were 1.0 100. 0μg/L with correlation coefficients above O. 9999. The mean recoveries were between 87.6% and 103.0%. Conclusion：This method can be applied for the determination of atrazine and bentazone in drinking water for its high sensitivity and high selectivity.
出处
《中国卫生检验杂志》
CAS
2008年第4期590-592,625,共4页
Chinese Journal of Health Laboratory Technology
