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Identification of the Sex of Earlier Embryos from Generic Hybrids of Chicken-Quail by Wpkci 被引量:1

Identification of the Sex of Earlier Embryos from Generic Hybrids of Chicken-Quail by Wpkci
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摘要 In this study, a protocol was developed to identify the sex of earlier embryos of chicken (♂)-quail (♀) hybrids and successfully tested the sex proportion of each period (66-120 h). We acquired cross bred eggs by artificial insemination, hatched them in the same batch according to the standard hatching condition of chicken, and collected earlier living embryos at 66, 72, 78, 84, 90, 96, 102, 108, 114, and 120 h randomly. We adopted RT-PCR protocol and multiple PCR, made the known sex quail as the external control, employed fl-actin as the internal control, and used primers that were designed according to conservative area of gene Wpkci of quail to identify the sex of earlier hybrid embryos. The results indicated that the primer of Wpkci can be used to identify the sex of hybrid embryos accurately; there were more male than female in earlier embryos, the sex proportion of earlier embryos compared with academic numerical value was significantly different (P〈0.01), and there was no difference between different periods (P〉0.05). In the present study, we concluded that a simple, fast, credible and stable protocol to identify the sex of earlier hybrids embryos had been established by using primer of Wpkci; in earlier embryos, the death rate of female was higher than that of male and there was no fluctuant peak. In this study, a protocol was developed to identify the sex of earlier embryos of chicken (♂)-quail (♀) hybrids and successfully tested the sex proportion of each period (66-120 h). We acquired cross bred eggs by artificial insemination, hatched them in the same batch according to the standard hatching condition of chicken, and collected earlier living embryos at 66, 72, 78, 84, 90, 96, 102, 108, 114, and 120 h randomly. We adopted RT-PCR protocol and multiple PCR, made the known sex quail as the external control, employed fl-actin as the internal control, and used primers that were designed according to conservative area of gene Wpkci of quail to identify the sex of earlier hybrid embryos. The results indicated that the primer of Wpkci can be used to identify the sex of hybrid embryos accurately; there were more male than female in earlier embryos, the sex proportion of earlier embryos compared with academic numerical value was significantly different (P〈0.01), and there was no difference between different periods (P〉0.05). In the present study, we concluded that a simple, fast, credible and stable protocol to identify the sex of earlier hybrids embryos had been established by using primer of Wpkci; in earlier embryos, the death rate of female was higher than that of male and there was no fluctuant peak.
出处 《Agricultural Sciences in China》 CAS CSCD 2008年第4期497-501,共5页 中国农业科学(英文版)
关键词 CHICKEN QUAIL hybrids embryos sex identification chicken quail hybrids embryos sex identification
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  • 1罗应荣,刘云海,朱化彬,朱成宽,朱志庆,宫云浩,吴时友,孙书华,蒋正军,邓必玉,罗国富,白俊品,武林源,赵振海.应用Y—DNA特异片段的PCR技术鉴定牛和绵羊胚胎性别及应用研究[J].黑龙江动物繁殖,1994,2(3):17-22. 被引量:7
  • 2Akira Ogawa,Irina Solovei,Nancy Hutchison,Yasushi Saitoh,Joh-E Ikeda,Herbert Macgregor,Shigeki Mizuno.Molecular characterization and cytological mapping of a non-repetitive DNA sequence region from the W chromosome of chicken and its use as a universal probe for sexing Carinatae birds[J].Chromosome Research.1997(2)
  • 3J. P. LeVine,L. E. Haley.Gene activation of alcohol dehydrogenase in Japanese quail and chicken-quail hybrid embryos[J].Biochemical Genetics (-).1975(7-8)
  • 4Peter G. Meyerhof,Leslie E. Haley.Ontogeny of lactate dehydrogenase isozymes in chicken-quail hybrid embryos[J].Biochemical Genetics (-).1975(1-2)
  • 5E. S. Leung,L. E. Haley.The ontogeny of phosphogluconate dehydrogenase and glucose 6-phosphate dehydrogenase in Japanese quails and chicken-quail hybrids[J].Biochemical Genetics.1974(3)
  • 6Ogawa A,Solovei I,Hutchison N,Saitoh Y,Ikeda J E,MacgregorH,Mizuno S.Molecular characterization andcytological mapping of a non-repetitive DNA sequence regionfrom the W chromosome of chicken and its use as an universalprobe for sexing carinatae birds[].Chromosome Research.1997
  • 7Pani P K.Genetic susceptibility of chicken × quail hybirdembryos to avian RNA tumour viruses[].The Journal ofGeneral Virology.1975
  • 8Shunsuke M,Jun O,Jun K,Tetsuya H,Shigeki M.PKCI-W forms a heterodimer with PKCI-Z and inhibits thebiological activities of PKCI-Z in vitro, supporting thepredicted role of PKCI-W in sex determination in birds[].Biochemistry.2006
  • 9Tang S S.The death mechanism of female embryos inchicken and quail hybridization[]..2006
  • 10Wang G Q,Tian W R,Duan X K,Sun D.The applicationof PCR in the sexual identification of early embryos of bovine[].Heilongjiang Journal of Animal Reproduction.2000

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