摘要
采用实验与生产结合的方法,研究了充气条件下几种饵料单胞藻的生长特性。结果表明,充气能大幅度提高4种饵料单胞藻的生长速度。在充气条件下,新月菱形藻、金藻3011、3012和扁藻的最大培养密度分别为2.957×107,1.7×107,2.355×107和3.5×106细胞/mL,分别是对照组最大培养密度的3.2倍,6.5倍,4.1倍和5倍;实验组(充气)与对照组(不充气)在细胞数目、特定生长率(SGR)、相对生长率(RGR)和绝对生长率(AGR)等方面均有显著差异。二级充气培养的藻液可以直接扩种到大池进行生产培养,能有效延长生长时间,显著提高生产培养效率。
Growth characteristics of 4 microalgae in bubble condition were studied using experiment combined with production method. The results showed that bubble incubation could improve the growth rate of 4 microalgae dramatically. Under bubble condition, the maximum cell density of N. closterium,L galbana Parke 3011 ,L galbana Parke 3012 and P. subcordiformis were 2.957× 10^7, 1.7 × 10^7 ,2. 355 × 10^7 and 3.5× 10^6 cell per milliliter, and which was 3.2, 6.5, 4.1 and 5 times of the control group under the same conditions respectively. The experiment group showed significant difference with the control one in cell number, special growth rate ( SGR), relative growth rate (RGR) and absolute growth rate (AGR). The air-charging incubation seed can be directly used for amplified culture. Air-charging incubation can effectively prolong the growth period of the algae cell, and remarkably enhance the production efficiency.
出处
《中国农业科技导报》
CAS
CSCD
2008年第2期94-99,共6页
Journal of Agricultural Science and Technology
基金
国家973项目(2006CB400608)
国家863计划项目(2006AA10Z414,2006AA100304)
中国博士后科学基金资助项目(2006039313)
山东省博士后择优基金项目(200603061)
国家自然科学基金项目(40776090,40706050,40706048,30700619)资助
关键词
单胞藻
充气培养
生长率
microalgae
air-charging incubation
growth rate