摘要
[目的]探究枯草杆菌诱导家蝇幼虫产生抗菌物质。[方法]研究枯草杆菌诱导的家蝇幼虫提取物的抗菌活性及其对温度、酸碱、离子强度的稳定性。[结果]枯草杆菌诱导的家蝇幼虫提取物对6种病原菌和工程菌大肠杆菌JM109均有抑制作用，其中对革兰氏阳性菌的抑制作用更强，诱导组的抗菌活性优于未诱导组。枯草杆菌诱导家蝇产生抗菌物质的具有很好的温度稳定性，在pH值4～10范围内都有较高的活性，但随着离子强度的升高抗菌活性下降。[结论]该研究为抗菌物质应用于饲料提供理论依据。
[Objective]The research aimed to study antibacterial substance produced by induction of the larvae of the housefly with Bacillus subtilis. [Method]The antibacterial activity of the extract of the housefly larvae induced by Bacillus subtilis and the stability to the temperature, pH and ion strength were studied. [Result]The extract of the housefly larvae induced by Bacillus subtilis was inhibitory to six pathogens and engineered bacteria E. coli JM 109, being more potent to Gram positive bacteria. The antibacterial effect of the induced group was higher than the non-induced. The antibacterial substance of housefly induced by Bacillus subtilis had good temperature stability, and remained high antibacterial activity in the range of pH 4 - 10, and gradually lost its activity with the ion strength increasing. [ Conclusion]The research provided theoretical basis for the application of antibacterial substance in the feedstuff.
出处
《安徽农业科学》
CAS
北大核心
2008年第10期4117-4118,共2页
Journal of Anhui Agricultural Sciences
基金
中国中心研究院所基础科学技术专项基金(2001DE-A20022)
关键词
家蝇
诱导
抗菌物质
Housefly （ Musca domest/ca）
Induction
Antibacterial substance
