摘要
应用基因工程技术，对本室已经克隆的日本血吸虫成虫３２ｋＤａ蛋白分子的ｃＤＮＡ片段进行ＰＣＲ扩增，扩增产物亚克隆入高效真核表达载体ｐＣＤ，构建成功裸露ＤＮＡ疫苗ｐＣＤＳｊ３２。ｐＣＤＳｊ３２在ＢＡＬＢ／ｃ小鼠骨骼肌细胞得到表达。表达产物可被小鼠抗３２ｋＤａ蛋白分子单抗识别。免疫荧光定位显示，表达产物不但存在于细胞浆，而且可结合于胞膜并被分泌至胞外。
The specific cDNA segment of 32kDa protein in Schistosoma japonicum was amplified with PCR. The product was subcloned into a high level eukaryotic expression vector pCD, then, naked DNA vaccine pCDSj32 was constructed. pCDSj32 was expressed in the skeletal muscular cells of BALB/c mice. The expressed products could be specially recognized by sera from rabbits infected with Schistosoma japonicum and monoclonal antibody to 32kDa protein. The expressed protein was not only located within the plasma of the cells, but also combined with the membranes and secreted out of the cells.
出处
《中国寄生虫病防治杂志》
CSCD
1997年第3期183-186,共4页
Chinese Journal of Parasitic Disease Control
关键词
日本血吸虫
成虫
血吸虫病
裸露DNA疫苗
免疫
Schistosoma japonicum , adult worm, 32kDa protein, naked DNA vaccine, expression
