摘要
探讨从藏灵菇中筛选的嗜热链球菌(Streptococcusthermophilus)所产胞外多糖(exopolysaccharide,EPS)对人结肠癌HCT-8细胞增殖的影响。采用CCK-8法测定EPS对HCT-8细胞生长的抑制率;形态学观察EPS对HCT-8细胞的杀伤作用;流式细胞术检测EPS对HCT-8细胞周期的影响。结果显示:EPS对HCT-8细胞增殖有明显的抑制作用,且呈时间-剂量依赖性;倒置显微镜下观察部分细胞脱壁、核碎裂、固缩,细胞悬浮坏死;细胞周期分析显示G1期细胞百分率增大。说明EPS通过直接杀伤人结肠癌HCT-8细胞,使细胞周期阻滞于G1期而抑制细胞增殖,从而发挥抗肿瘤作用。
To investigate the proliferation effect of exopolysaccharide（EPS）produced from Streptococcus thermophilus of Tibetan kefir on human colon cancer HCT-8 ceils. CCK-8 assay was used to examine the effect of EPS in anti-proliferation, killing effect of EPS was evaluated through morphology identification combined with fluorescence microscopy observation, the cell cycle pattern was studied by flow cytometry. CCK-8 assay showed that the proliferation of HCT-8 ceils was inhibited apparently after treatment with EPS, and in a time- and dose-dependent manner. EPS treatment resulted in morphological changes, including cell shrinkage, membrane blebbing, nuclear condensation, boundary aggregation or split, the necrotic cells increased along with the elevation of EPS concentration, and leading to cell cycle arrest at G1 phase. EPS showed that its anti- turner effect by way of direct killing effects and make cells arresting the G1 phase to inhibiting the cell growth.
出处
《食品科学》
CAS
CSCD
北大核心
2008年第4期405-408,共4页
Food Science
基金
北京市自然科学基金资助项目(5062006)
关键词
嗜热链球菌
胞外多糖
人结肠癌细胞
增殖抑制
细胞周期
Streptococcus thermophilus
exopolysaccharide
human colon cancer ceils
anti-proliferation
cell cycle
