摘要
目的建立VX2乳腺癌兔的阿霉素致的心脏毒性模型。方法先建立兔VX2乳腺癌模型。2周后,将接种VX2乳腺癌成功模型兔随机分为阿霉素(ADR)组和对照组,阿霉素组10只,雌雄各5只,对照组8只,雌雄各4只。ADR组,每周1次于兔耳缘静脉注射ADR(用生理盐水配制成1mg/ml)2mg/kg,共6次。对照组,每周1次于耳缘静脉推注生理盐水每次2ml/kg,共6次。比较左心室射血分数的变化,并作心脏和肿块的病理切片,观察其组织学变化,记录动物的生存时间。结果模型成功率为90%,两组左心室射血分数(LVEF)差异有统计学意义(P<0.05),阿霉素组的心脏经病理检查证实为有心肌病变,病理学结果证实符合心肌病样改变,肿块经病理检验证实为鳞状细胞癌。结论成功建立了VX2乳腺癌兔的阿霉素致心脏毒性模型。
Objective To establish adriamycin-induced Cardiactoxicity model in rabbit Xenograft Model of Breast Cancer VX2. Methods To establish Xenograft Model of Breast Cancer VX2 in rabbit. After 2 weeks,The rabbits with VX2 breast carcinoma were randomly divided into the Adriamycin treatment group （10 cases）and the control group （8 cases）. The Treatment group was given Adriamycin 2 mg/kg every week into auricular vein of ears for consecutive 6 weeks after the first injection. The control group was given normal saline respectively. The Changes of left ventricular ejection fraction（LVEF） were compared between the two groups. Hearts and Tumors were harvested after that and analyzed by histological staining. Tumor-bearing survival time was recorded. Results The successful rate of the model group was 90. There was a significant difference in LVEF% between the two groups （ P 〈 0. 05 ）. Pathological examination demonstrated that the cardiac muscular tissue of the model group were harmed, which characteristic cconsistent with cardiomyopathy. Tumors were conformaed to the feature of squamous cell carcinoma . Conclusion Adriamycin-induced Cardiactoxicity model in rabbit Xenograft Model of Breast Cancer VX2, which would provide a considerable aninel model for investigation of clinic ,was established successfully.
出处
《中国现代药物应用》
2008年第9期5-7,共3页
Chinese Journal of Modern Drug Application
