摘要
采用分光光度法和扫描电子显微镜法对桑叶多酚氧化酶(PPO)的结构、活性以及不同抑制剂的抑制效果进行研究。结果表明:桑叶多酚氧化酶为纤维状蛋白;它的最适pH值为7.0;最适温度为40℃;在相同抑制剂浓度下,总体抑制效果为:抗坏血酸>亚硫酸氢钠>柠檬酸>氯化镁>氯化钠。
The characteristics of polyphenol oxidase （PPO） in mulberry leaf were studied by Spectrophotometer method and scanning electron microscopy. The inhibiting effects of different inhibitors on the activity of PPO were studied as well. The result showed that PPO of mulberry leaf are fibrous protein; the optimum pH and temperature are 7.0 and 40 ℃ for PPO of mulberry leaf respectively, in the same concentrations of inhibitor, the inhibiting effects as follow： ascorbic acid〉sodium hydrogen sulfite〉citric acid〉magnesium chloride〉sodium chloride.
出处
《食品研究与开发》
CAS
北大核心
2008年第5期11-15,共5页
Food Research and Development
基金
广西自然科学基金项目(桂科自0542016)
关键词
桑叶
多酚氧化酶
纯化
活性
mulberry leaf
polyphenol oxidase
purification
activity
